Wang Fei, Lin Qiao, Shi Xueqin, Li Yunfang, Deng Pengyu, Zhang Yuping, Hu Deyu
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, PR China.
Food Chem X. 2022 Jul 8;15:100383. doi: 10.1016/j.fochx.2022.100383. eCollection 2022 Oct 30.
An efficient trace detection method for the determination of residues of the glufosinate enantiomers and metabolites in zebrafish by HPLC-Q-Exactive Orbitrap Mass Spectrometry was developed. After the purification of dichloromethane and Oasis PRiME HLB SPE column, the recovery ranges from 77% to 104%, with RSD < 10.03%. The limits of quantitation in zebrafish were 0.006-0.02 mg/kg. The results revealed zebrafish absorbed glufosinate slowly, reaching a steady state in 10-14 days, and the bioaccumulation factor (BCF) of D/L-glufosinate-ammonium was less than 0.3. L-glufosinate-ammonium accumulated preferentially in zebrafish. The residue of the metabolite -acetyl glutamate (NAG) was smaller than that of 3-methyl phosphonic acid (MPP). D/L-glufosinate-ammonium had an elimination half-life of less than 2.3 days during the elimination phase. The bioaccumulation and elimination behavior of glufosinate-ammonium in zebrafish aquatic system was shown in this work, which offered scientific data for assessing the food safety of rac-glufosinate-ammonium and glufosinate-P (pure L-glufosinate-ammonium) in fish.
建立了一种高效的痕量检测方法，用于通过高效液相色谱- Q- Exactive轨道阱质谱法测定斑马鱼中草铵膦对映体及其代谢物的残留量。经二氯甲烷和Oasis PRiME HLB固相萃取柱净化后，回收率在77%至104%之间，相对标准偏差<10.03%。斑马鱼中的定量限为0.006 - 0.02 mg/kg。结果表明，斑马鱼对草铵膦的吸收缓慢，在10 - 14天内达到稳态，D/L-草铵膦铵的生物累积因子（BCF）小于0.3。L-草铵膦铵优先在斑马鱼中累积。代谢物N-乙酰谷氨酸（NAG）的残留量小于3-甲基膦酸（MPP）。在消除阶段，D/L-草铵膦铵的消除半衰期小于2.3天。本研究展示了草铵膦铵在斑马鱼水生系统中的生物累积和消除行为，为评估消旋草铵膦铵和草铵膦-P（纯L-草铵膦铵）在鱼类中的食品安全提供了科学数据。
