Ghoshal Goutam, Oelze Michael L
Bioacoustic Research Laboratory, Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 405 North Mathews, Urbana, Illinois 61801, USA
J Acoust Soc Am. 2015 Dec;138(6):EL493-7. doi: 10.1121/1.4936644.
Ultrasound-stimulated microbubbles (MBs) were demonstrated to enhance cell kill from hyperthermia. Definity MBs were injected into wells containing 4T1 cells in culture media and scanned with 1-MHz ultrasound, an exposure duration of 30 s and a negative pressure of 0.5 or 1.3 MPa. Some cell samples were placed in a water bath heated to 42 °C for 5 min. Cell death was quantified. When combining MBs, ultrasound at 1.3 MPa and hyperthermia, more than 58.8% ± 7.21% of cells were nonviable. When exposed to hyperthermia alone or exposure to MBs and ultrasound but no hyperthermia, cell death was less than 10.1% ± 6.96% and 30.1% ± 10.8%, respectively.
超声刺激微泡（MBs）已被证明可增强热疗对细胞的杀伤作用。将Definity微泡注入含有4T1细胞的培养基孔中，并用1MHz超声进行扫描，暴露持续时间为30秒，负压为0.5或1.3MPa。一些细胞样本置于加热至42°C的水浴中5分钟。对细胞死亡情况进行定量分析。当联合使用微泡、1.3MPa超声和热疗时，超过58.8%±7.21%的细胞失去活性。当单独进行热疗或暴露于微泡和超声但无热疗时，细胞死亡率分别低于10.1%±6.96%和30.1%±10.8%。
