Elkholy Yasmine Samy, Hegab Asmaa Sayed, Ismail Dalia Kadry, Hassan Reem Mostafa
Department of Medical Microbiology and Immunology, Cairo University, Cairo, 11562, Egypt.
Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, 11562, Egypt.
J Microbiol. 2016 Jan;54(1):39-43. doi: 10.1007/s12275-016-5530-0. Epub 2016 Jan 5.
Endoscopes are a common source of outbreaks of healthcare-associated infections. It is therefore important to identify high-level disinfectants capable of eliminating or killing all vegetative bacteria, mycobacteria, and viruses. Aldehydebased disinfectants are most commonly used in clinical practice but resistance has recently been detected and side effects associated with these disinfectants are well documented. In this study, we evaluated Virusolve+® EDS, a novel quaternary ammonium compound formulation supplied by Amity international, against Mycobacterium bovis (ATCC-27289), hepatitis C virus (HCV)-positive serum and hepatitis B surface antigen-positive serum. We also compared its efficacy against Cidex® (glutaraldehyde 2%), an aldehyde-based disinfectant. M. bovis showed no growth after 10 weeks with either Virusolve+® or Cidex®. Virusolve+® achieved a 10(4)- fold reduction in the initial 10(6) HCV load under clean conditions (without red blood cells) for 20 min, whereas Cidex® achieved this reduction under clean and dirty conditions (without and with red blood cells, respectively) after both 10 and 20 min. Both Virusolve+® and Cidex® were able to eradicate hepatitis B virus (HBV) infectivity under clean conditions after 10 and 20 min, whereas under dirty conditions they were only able to eradicate virus infectivity after 20 min. Virusolve+® EDS when compared with Cidex® showed equal mycobactericidal activity completely eradicating M. bovis. However, both showed comparable virucidal activity against HBV, which was more effective under clean conditions, emphasizing the importance of the cleaning step in endoscope reprocessing. Cidex® was more effective at eradicating HCV under dirty conditions after a short contact time.
内窥镜是医疗保健相关感染暴发的常见源头。因此，识别能够消灭或杀灭所有繁殖体细菌、分枝杆菌和病毒的高水平消毒剂很重要。基于醛的消毒剂在临床实践中最常用，但最近已检测到耐药性，并且与这些消毒剂相关的副作用有充分记录。在本研究中，我们评估了由友好国际公司提供的新型季铵化合物配方Virusolve+® EDS对牛分枝杆菌（ATCC - 27289）、丙型肝炎病毒（HCV）阳性血清和乙型肝炎表面抗原阳性血清的效果。我们还比较了它与基于醛的消毒剂Cidex®（2%戊二醛）的功效。使用Virusolve+® 或Cidex® 处理后，牛分枝杆菌在10周后均未生长。在清洁条件下（无红细胞），Virusolve+® 在20分钟内使初始的10⁶ HCV载量降低了10⁴倍，而Cidex® 在清洁和污染条件下（分别无和有红细胞）在10分钟和20分钟后均实现了这种降低。在清洁条件下处理10分钟和20分钟后，Virusolve+® 和Cidex® 均能够消除乙型肝炎病毒（HBV）的传染性，而在污染条件下，它们仅在20分钟后能够消除病毒传染性。与Cidex® 相比，Virusolve+® EDS表现出同等的杀分枝杆菌活性，能完全根除牛分枝杆菌。然而，两者对HBV均表现出相当的杀病毒活性，在清洁条件下更有效，这强调了内窥镜再处理中清洁步骤的重要性。在污染条件下短时间接触后，Cidex® 在根除HCV方面更有效。
