Araújo Paula A, Lemos Madalena, Mergulhão Filipe, Melo Luís, Simões Manuel
LEPAE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
Int J Food Sci. 2013;2013:237581. doi: 10.1155/2013/237581. Epub 2013 Sep 14.
Standard cleaning processes may not remove all the soiling typically found in food industry, such as carbohydrates, fats, or proteins. Contaminants have a high impact in disinfection as their presence may reduce the activity of disinfectants. The influence of alginic acid, bovine serum albumin, yeast extract, and humic acids was assessed on the antimicrobial activities of benzalkonium chloride and cetyltrimethyl ammonium bromide against Bacillus cereus vegetative cells and Pseudomonas fluorescens. The bacteria (single and consortium) were exposed to surfactants (single and combined) in the absence and presence of potential disinfection interfering substances. The antimicrobial effects of the surfactants were assessed based on the bacterial respiratory activity measured by oxygen uptake rate due to glucose oxidation. The tested surfactants were efficient against both bacteria (single and consortium) with minimum bactericidal concentrations ranging from 3 to 35 mg·L(-1). The strongest effect was caused by humic acids that severely quenched antimicrobial action, increasing the minimum bactericidal concentration of the surfactants on P. fluorescens and the consortium. The inclusion of the other interfering substances resulted in mild interferences in the antibacterial activity. This study clearly demonstrates that humic acids should be considered as an antimicrobial interfering substance in the development of disinfection strategies.
标准清洁流程可能无法清除食品工业中常见的所有污垢,如碳水化合物、脂肪或蛋白质。污染物对消毒有很大影响,因为它们的存在可能会降低消毒剂的活性。评估了海藻酸、牛血清白蛋白、酵母提取物和腐殖酸对苯扎氯铵和十六烷基三甲基溴化铵对蜡状芽孢杆菌营养细胞和荧光假单胞菌的抗菌活性的影响。在不存在和存在潜在消毒干扰物质的情况下,将细菌(单一菌和混合菌)暴露于表面活性剂(单一和组合)中。基于因葡萄糖氧化导致的氧气摄取率所测量的细菌呼吸活性来评估表面活性剂的抗菌效果。所测试的表面活性剂对两种细菌(单一菌和混合菌)均有效,最低杀菌浓度范围为3至35mg·L⁻¹。腐殖酸造成的影响最为强烈,它严重抑制了抗菌作用,提高了表面活性剂对荧光假单胞菌和混合菌的最低杀菌浓度。其他干扰物质的存在对抗菌活性产生了轻微干扰。这项研究清楚地表明,在制定消毒策略时,应将腐殖酸视为一种抗菌干扰物质。