Liang Xiao-Rui, Miao Feng-Ping, Song Yin-Ping, Guo Zhan-Yong, Ji Nai-Yun
a Yantai Institute of Coastal Zone Research , Chinese Academy of Sciences , Yantai , China.
b Department of Basic Sciences , Naval Aeronautical and Astronautical University , Yantai , China.
Nat Prod Res. 2016 Jul;30(14):1605-10. doi: 10.1080/14786419.2015.1126264. Epub 2016 Jan 4.
One new diterpene, trichocitrin (1), and four known secondary metabolites, nafuredin (2), 5-hydroxy-2,3-dimethyl-7-methoxychromone (3), 24-methylenecycloartanol (4) and citrostadienol (5), were isolated from the culture of marine brown alga-endophytic Trichoderma citrinoviride cf-27. Trichocitrin (1) represents the first Trichoderma-derived and furan-bearing fusicoccane diterpene, and its structure and relative configuration were identified by analysis of 1D/2D NMR and mass spectroscopic data. Compounds 1 and 2 exhibited 8.0- and 9.5-mm inhibition zones, respectively, against Escherichia coli at 20 μg/disc and 54.1 and 36.7% growth inhibition, respectively, of Prorocentrum donghaiense at 80 μg/mL.
从海洋褐藻内生黄绿木霉cf - 27的培养物中分离出一种新的二萜类化合物——曲霉柠檬素（1），以及四种已知的次生代谢产物，萘呋地定（2）、5 - 羟基 - 2,3 - 二甲基 - 7 - 甲氧基色酮（3）、24 - 亚甲基环阿尔廷醇（4）和柠檬甾二烯醇（5）。曲霉柠檬素（1）是首个源自木霉且含有呋喃的镰刀烷二萜类化合物，通过一维/二维核磁共振和质谱数据分析确定了其结构和相对构型。化合物1和2在浓度为20μg/圆片时，对大肠杆菌的抑菌圈直径分别为8.0mm和9.5mm；在浓度为80μg/mL时，对东海原甲藻的生长抑制率分别为54.1%和36.7%。
