Vega Daniel L, Lodge Patrick, Vivero-Escoto Juan L
Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
The Center for Biomedical Engineering and Science, University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
Int J Mol Sci. 2015 Dec 31;17(1):56. doi: 10.3390/ijms17010056.
The development of stimulus-responsive photosensitizer delivery systems that carry a high payload of photosensitizers is of great importance in photodynamic therapy. In this study, redox-responsive polysilsesquioxane nanoparticles (PSilQNPs) built by a reverse microemulsion approach using 5,10,15,20-tetrakis(carboxyphenyl) porphyrin (TCPP) silane derivatives as building blocks, were successfully fabricated. The structural properties of TCPP-PSilQNPs were characterized by dynamic light scattering (DLS)/ζ-potential, scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). The photophysical properties were determined by UV-vis and fluorescence spectroscopy. The quantity of singlet oxygen generated in solution was measured using 1,3-diphenylisobenzofuran. The redox-responsive release of TCPP molecules was successfully demonstrated in solution in the presence of a reducing agent. The internalization of TCPP-PSilQNPs in cancer cells was investigated using laser scanning confocal microscopy. Phototoxicity experiments in vitro showed that the redox-responsive TCPP-PSilQNPs exhibited an improved phototherapeutic effect on cervical cancer cells compared to a non-responsive TCPP-PSilQNP control material.
开发能够携带高负载光敏剂的刺激响应型光敏剂递送系统在光动力疗法中具有重要意义。在本研究中，以5,10,15,20-四（羧基苯基）卟啉（TCPP）硅烷衍生物为结构单元，通过反相微乳液法成功制备了氧化还原响应型聚倍半硅氧烷纳米颗粒（PSilQNPs）。采用动态光散射（DLS）/ζ电位、扫描电子显微镜（SEM）和热重分析（TGA）对TCPP-PSilQNPs的结构性质进行了表征。通过紫外可见光谱和荧光光谱测定了其光物理性质。使用1,3-二苯基异苯并呋喃测定溶液中产生的单线态氧的量。在还原剂存在的情况下，成功地在溶液中证明了TCPP分子的氧化还原响应释放。使用激光扫描共聚焦显微镜研究了TCPP-PSilQNPs在癌细胞中的内化情况。体外光毒性实验表明，与无响应的TCPP-PSilQNP对照材料相比，氧化还原响应型TCPP-PSilQNPs对宫颈癌细胞表现出更好的光治疗效果。
