Vivero-Escoto Juan L, Elnagheeb Maram
Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
Center for Biomedical Engineering and Science, University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
Nanomaterials (Basel). 2015 Dec 16;5(4):2302-2316. doi: 10.3390/nano5042302.
Mesoporous silica nanoparticles (MSNs) have been synthesized and loaded with both aluminum chloride phthalocyanine (AlClPc) and cisplatin as combinatorial therapeutics for treating cancer. The structural and photophysical properties of the MSN materials were characterized by different spectroscopic and microscopic techniques. Intracellular uptake and cytotoxicity were evaluated in human cervical cancer (HeLa) cells by confocal laser scanning microscopy (CLSM) and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assays, respectively. The CLSM experiments showed that the MSN materials can be readily internalized in HeLa cells. The cytotoxic experiments demonstrated that, after light exposure, the combination of both AlClPc and cisplatin compounds in the same MSN platform potentiate the toxic effect against HeLa cells in comparison to the control AlClPc-MSN and cisplatin-MSN materials. These results show the potential of using MSN platforms as nanocarriers for combination photodynamic and chemotherapies to treat cancer.
介孔二氧化硅纳米颗粒（MSNs）已被合成，并负载了酞菁氯化铝（AlClPc）和顺铂，作为治疗癌症的联合疗法。通过不同的光谱和显微镜技术对MSN材料的结构和光物理性质进行了表征。分别通过共聚焦激光扫描显微镜（CLSM）和3-（4,5-二甲基噻唑-2-基）-5-（3-羧甲氧基苯基）-2-（4-磺基苯基）-2H-四唑（MTS）测定法评估了人宫颈癌（HeLa）细胞中的细胞内摄取和细胞毒性。CLSM实验表明，MSN材料可以很容易地被HeLa细胞内化。细胞毒性实验表明，与对照AlClPc-MSN和顺铂-MSN材料相比，在相同的MSN平台中同时存在AlClPc和顺铂化合物，光照后对HeLa细胞的毒性作用增强。这些结果表明，使用MSN平台作为纳米载体进行联合光动力和化疗治疗癌症具有潜力。
