Prestegard Siv Kristin, Erga Svein Rune, Steinrücken Pia, Mjøs Svein Are, Knutsen Gjert, Rohloff Jens
Bergen Marine Biobank, Department of Biology, University of Bergen, Thormøhlensgate 53A/B, N-5020 Bergen, Norway.
Applied Biotechnology, Uni Research Environment, Thormøhlensgate 49B, N-5006 Bergen, Norway.
Mar Drugs. 2015 Dec 30;14(1):9. doi: 10.3390/md14010009.
We have searched for special characteristics in growth, protein expression, fatty acids and volatile organic compounds (VOCs) in a local Phaeodactylum tricornutum Bohlin strain (Bergen Marine Biobank), by comparing it with a common accession strain (CCAP). Differences in growth and expressed proteins were detected between the BMB strain and the CCAP strain, and the BMB strain reached the highest cell densities under the given growth conditions. Fatty acid (FA) analyses showed highest relative eicosapentaenoic acid (EPA) levels in the exponential phase (25.73% and 28.31%), and highest levels of palmitoleic acid (16:1 n-7) in the stationary phase (46.36% and 43.66%) in the BMB and CCAP strain, respectively. The most striking finding of the VOCs analyses was the relatively high levels of ectocarpene, 6-((1E)-butenyl)-1,4-cycloheptadiene, hormosirene, and desmarestene and structurally related compounds, which were exclusively detected in the BMB strain. Many of the VOCs detected in the CCAP and, in particular, in the BMB strain have been reported as antimicrobial agents. We suggest that the array of pheromones and antimicrobial substances could be part of an allelopathic strategy of the BMB strain, dominated by oval cells, thus reflecting the benthic life stage of this morphological form. These findings show the potential for bioactive metabolites in the BMB strain.
我们通过将本地三角褐指藻菌株（卑尔根海洋生物样本库）与一个常见保藏菌株（CCAP）进行比较，研究了其在生长、蛋白质表达、脂肪酸和挥发性有机化合物（VOCs）方面的特殊特征。在BMB菌株和CCAP菌株之间检测到了生长和表达蛋白质的差异，并且BMB菌株在给定的生长条件下达到了最高细胞密度。脂肪酸（FA）分析表明，在指数生长期，BMB菌株和CCAP菌株中二十碳五烯酸（EPA）的相对含量最高（分别为25.73%和28.31%）；在稳定期，BMB菌株和CCAP菌株中棕榈油酸（16:1 n-7）的含量最高（分别为46.36%和43.66%）。VOCs分析最显著的发现是，在BMB菌株中专门检测到了相对较高含量的外果皮素、6-((1E)-丁烯基)-1,4-环庚二烯、激素紫菜素和去甲马尾藻烯以及结构相关化合物。在CCAP菌株，特别是在BMB菌株中检测到的许多VOCs已被报道为抗菌剂。我们认为，一系列信息素和抗菌物质可能是BMB菌株化感策略的一部分，该菌株以椭圆形细胞为主，从而反映了这种形态形式的底栖生活阶段。这些发现表明BMB菌株中生物活性代谢物具有潜在价值。
