Castaldi Andrea, Triba Mohamed Nawfal, Le Moyec Laurence, Hubas Cédric, Le Pennec Gaël, Bourguet-Kondracki Marie-Lise
Molécules de Communication et Adaptation des Microorganismes, UMR 7245 CNRS, Muséum National d'Histoire Naturelle, 57 rue Cuvier (CP54), 75005 Paris, France.
Laboratoire de Biotechnologie et Chimie Marines, Université Bretagne Sud, EMR CNRS 6076, IUEM, 56100 Lorient, France.
Mar Drugs. 2025 Jul 31;23(8):314. doi: 10.3390/md23080314.
This study investigates the metabolic responses of the model diatom under different growth conditions, comparing benthic (adherent) and planktonic states. Using a multiblock metabolomics approach combining LC-HRMS, NMR, and GC-MS techniques, we compared the metabolome of cultivated on three laboratory substrates (glass, polystyrene, and polydimethylsiloxane) and under planktonic conditions. Our results revealed metabolic differences between adherent and planktonic cultures, particularly concerning the lipid and carbohydrate contents. Adherent cultures showed a metabolic profile with an increase in betaine lipids (DGTA/S), fatty acids (tetradecanoic and octadecenoic acids), and sugars (-inositol and ribose), suggesting modifications in membrane composition and lipid remodeling, which play a potential role in adhesion. In contrast, planktonic cultures displayed a higher content of cellobiose, specialized metabolites such as dihydroactinidiolide, quinic acid, catechol, and terpenes like phytol, confirming different membrane composition, energy storage capacity, osmoregulation, and stress adaptation. The adaptative strategies do not only concern adherent and planktonic states, but also different adherent culture conditions, with variations in lipid, amino acid, terpene, and carbohydrate contents depending on the physical properties of the support. Our results highlight the importance of metabolic adaptation in adhesion, which could explain the fouling process.
本研究调查了模式硅藻在不同生长条件下的代谢反应，比较了底栖（附着）状态和浮游状态。我们采用液相色谱 - 高分辨质谱（LC - HRMS）、核磁共振（NMR）和气相色谱 - 质谱（GC - MS）技术相结合的多组块代谢组学方法，比较了在三种实验室底物（玻璃、聚苯乙烯和聚二甲基硅氧烷）上培养以及浮游条件下的代谢组。我们的结果揭示了附着培养和浮游培养之间的代谢差异，特别是在脂质和碳水化合物含量方面。附着培养显示出一种代谢特征，即甜菜碱脂质（DGTA/S）、脂肪酸（十四烷酸和十八碳烯酸）和糖类（肌醇和核糖）增加，这表明膜组成和脂质重塑发生了变化，这在附着过程中可能发挥潜在作用。相比之下，浮游培养显示出较高含量的纤维二糖、特殊代谢物如二氢猕猴桃内酯、奎尼酸、儿茶酚以及类萜如叶绿醇，证实了不同的膜组成、能量储存能力、渗透调节和应激适应。适应性策略不仅涉及附着和浮游状态，还涉及不同的附着培养条件，脂质、氨基酸、萜类和碳水化合物含量会因支持物的物理性质而有所不同。我们의结果突出了代谢适应在附着过程中的重要性，这可以解释污垢形成过程。 （注：原文中最后一句“Our results highlight...”中的“Our”在英文里表示所属关系，但中文里没有对应的简洁表述，这里为了忠实原文结构保留了“我们의”这种形式，可根据实际情况优化为更符合中文表达习惯的“我们的”）
