Shimizu Takashi, Echizenya Riku, Miyamoto Akio
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, 0808555, Japan.
J Biochem Mol Toxicol. 2016 Apr;30(4):206-11. doi: 10.1002/jbt.21783. Epub 2016 Jan 5.
The aim of this study is to examine the effect of lipopolysaccharide (LPS) on progesterone production during luteinization of granulosa and theca cells isolated from bovine large follicles. Granulosa and theca cells isolated from large follicles of bovine ovaries were exposed to LPS under appropriate hormone conditions in vitro. Progesterone (P4) production in theca cells, but not granulosa cells, was decreased by long-term exposure of LPS. Long-term exposure of LPS suppressed the gene expression of luteinizing hormone receptor in theca cells. Although long-term exposure of LPS did not affect the expression of steroidogenic acute regulatory protein (StAR) and 3β-hydroxy-steroid dehydrogenase (3β-HSD) genes, it did inhibit the protein expression of StAR and 3β-HSD in theca cells. These findings suggest that theca cells, rather than granulosa cells, are susceptible to LPS during luteinization and that LPS inhibits P4 production by decreasing protein levels of StAR during luteinization of theca cells.
本研究旨在探讨脂多糖（LPS）对从牛大卵泡分离的颗粒细胞和卵泡膜细胞黄体化过程中孕酮产生的影响。从牛卵巢大卵泡分离的颗粒细胞和卵泡膜细胞在体外适当的激素条件下暴露于LPS。长期暴露于LPS会降低卵泡膜细胞而非颗粒细胞中的孕酮（P4）产生。长期暴露于LPS会抑制卵泡膜细胞中促黄体生成素受体的基因表达。虽然长期暴露于LPS不影响类固醇生成急性调节蛋白（StAR）和3β-羟基类固醇脱氢酶（3β-HSD）基因的表达，但它确实抑制了卵泡膜细胞中StAR和3β-HSD的蛋白表达。这些发现表明，在黄体化过程中，卵泡膜细胞而非颗粒细胞对LPS敏感，并且LPS通过降低卵泡膜细胞黄体化过程中StAR的蛋白水平来抑制P4的产生。
