一种基于DNA四面体的分子信标用于活细胞中肿瘤相关mRNA的检测。
A DNA tetrahedron-based molecular beacon for tumor-related mRNA detection in living cells.
作者信息
Xie Nuli, Huang Jin, Yang Xiaohai, Yang Yanjing, Quan Ke, Wang He, Ying Le, Ou Min, Wang Kemin
机构信息
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China.
出版信息
Chem Commun (Camb). 2016 Feb 7;52(11):2346-9. doi: 10.1039/c5cc09980c.
Due to its low cytotoxicity, high resistance to enzymatic degradation, and cellular permeability, a DNA tetrahedron-based molecular beacon (DTMB) is designed for tumor-related TK1 mRNA detection in living cells, where the target sequence can induce the tetrahedron from contraction to extension, resulting in fluorescence restoration.
由于其低细胞毒性、对酶降解的高抗性和细胞通透性，设计了一种基于DNA四面体的分子信标（DTMB）用于活细胞中肿瘤相关TK1 mRNA的检测，其中靶序列可诱导四面体从收缩状态转变为伸展状态，从而导致荧光恢复。
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