Xie Nuli, Huang Jin, Yang Xiaohai, Yang Yanjing, Quan Ke, Wang He, Ying Le, Ou Min, Wang Kemin
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China.
Chem Commun (Camb). 2016 Feb 7;52(11):2346-9. doi: 10.1039/c5cc09980c.
Due to its low cytotoxicity, high resistance to enzymatic degradation, and cellular permeability, a DNA tetrahedron-based molecular beacon (DTMB) is designed for tumor-related TK1 mRNA detection in living cells, where the target sequence can induce the tetrahedron from contraction to extension, resulting in fluorescence restoration.
由于其低细胞毒性、对酶降解的高抗性和细胞通透性，设计了一种基于DNA四面体的分子信标（DTMB）用于活细胞中肿瘤相关TK1 mRNA的检测，其中靶序列可诱导四面体从收缩状态转变为伸展状态，从而导致荧光恢复。
