Montes-García J F, Vaca S, Vazquez-Cruz C, Soriano-Vargas E, Aguilar-Romero F, Blackall P J, Negrete-Abascal E
Carrera de Biología, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios # 1, Los Reyes Iztacala, 54090, Tlalnepantla, Estado de México, Mexico.
Centro de Investigaciones en Ciencias Microbiológicas, Instituto de ciencias, BUAP, Apdo. Postal 1622, 72560, Puebla, Mexico.
Curr Microbiol. 2016 Apr;72(4):450-6. doi: 10.1007/s00284-015-0969-5. Epub 2016 Jan 4.
Gallibacterium anatis has the ability to hemagglutinate rabbit erythrocytes; however, no bacterial component has yet been associated with this function. In the present work, a protein of approximately 65 kDa with hemagglutinating activity for glutaraldehyde-fixed chicken erythrocytes was purified by ion interchange chromatography from G. anatis F149(T) secreted proteins. The protein was recognized by a rabbit polyclonal serum against a hemagglutinin from Avibacterium paragallinarum. The 65 kDa purified protein presented identity with a G. anatis filamentous hemagglutinin by mass spectrometric analysis. As well, the bacterial surface of G. anatis was labeled by immune gold assays using a polyclonal serum against the 65-kDa protein. A similar protein was recognized in four other G. anatis strains by immunoblots using the same antiserum. The protein binds sheep or pig biotinylated fibrinogen, suggesting an interaction with basement membrane eukaryotic cells components, and the protein is present in G. anatis biofilms. Overall, the results suggest that the 65 kDa hemagglutinin is a common antigen and a potential virulence factor in G. anatis.
鸭源鸡杆菌具有凝集兔红细胞的能力；然而，尚未发现有细菌成分与该功能相关。在本研究中，通过离子交换色谱法从鸭源鸡杆菌F149(T)分泌蛋白中纯化出一种对戊二醛固定的鸡红细胞具有血凝活性的约65 kDa蛋白质。该蛋白质可被抗副鸡禽杆菌血凝素的兔多克隆血清识别。通过质谱分析，纯化的65 kDa蛋白质与鸭源鸡杆菌丝状血凝素具有同一性。同样，利用抗65 kDa蛋白质的多克隆血清通过免疫金测定法标记了鸭源鸡杆菌的细菌表面。使用相同抗血清通过免疫印迹在其他四株鸭源鸡杆菌菌株中也识别出了类似的蛋白质。该蛋白质结合绵羊或猪生物素化纤维蛋白原，表明其与基底膜真核细胞成分存在相互作用，并且该蛋白质存在于鸭源鸡杆菌生物膜中。总体而言，结果表明65 kDa血凝素是鸭源鸡杆菌中的一种共同抗原和潜在毒力因子。
