Mustafa Hesham N, El Awdan Sally A, Hegazy Gehan A, Abdel Jaleel Gehad A
Department of Anatomy, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
Department of Pharmacology, National Research Center, Cairo, Egypt.
Indian J Pharmacol. 2015 Nov-Dec;47(6):649-56. doi: 10.4103/0253-7613.169588.
The study aims to evaluate the protective effects of coenzyme Q10 (CoQ10) and Cynara scolymus L (CS) on doxorubicin (dox)-induced toxicity.
Sixty male rats were divided into six groups. Group 1 as a control. Group 2 received dox (10 mg/kg) intraperitoneally. Group 3 received CoQ10 (200 mg/kg). Group 4 received CS (500 mg/kg). Group 5 received CoQ10 (200 mg/kg) and dox (10 mg/kg). Group 6 received CS (500 mg/kg) and dox (10 mg/kg). The rats were then evaluated biochemically and immunohistochemically.
Dox produced a significant deterioration of hepatic and renal functional parameters. Moreover, an upsurge of oxidative stress and nitrosative stress markers. The expression of alpha-smooth muscle actin (α-SMA) was increased and proliferating cell nuclear antigen (PCNA) expression was decreased. Administration of CoQ10 and CS resulted in a significant improvement of hepatic and renal functional parameters, and an improvement of both α-SMA and PCNA.
It is concluded that pretreatment with CoQ10 and CS is associated with up-regulation of favorable protective enzymes and down-regulation of oxidative stress. That can be advised as a supplement to dox-treated patients.
本研究旨在评估辅酶Q10（CoQ10）和洋蓟（CS）对阿霉素（阿霉素）诱导的毒性的保护作用。
将60只雄性大鼠分为6组。第1组作为对照组。第2组腹腔注射阿霉素（10mg/kg）。第3组给予CoQ10（200mg/kg）。第4组给予CS（500mg/kg）。第5组给予CoQ10（200mg/kg）和阿霉素（10mg/kg）。第6组给予CS（500mg/kg）和阿霉素（10mg/kg）。然后对大鼠进行生化和免疫组化评估。
阿霉素导致肝脏和肾脏功能参数显著恶化。此外，氧化应激和亚硝化应激标志物激增。α平滑肌肌动蛋白（α-SMA）表达增加，增殖细胞核抗原（PCNA）表达降低。给予CoQ10和CS可显著改善肝脏和肾脏功能参数，并改善α-SMA和PCNA。
得出结论，CoQ10和CS预处理与上调有利的保护酶和下调氧化应激有关。这可以建议作为阿霉素治疗患者的补充。
