顽固性癫痫患者颞叶新皮质前部中的棘皮动物微管相关蛋白样蛋白5
Echinoderm microtubule-associated protein -like protein 5 in anterior temporal neocortex of patients with intractable epilepsy.
作者信息
Sun Ji-Jun, Huang Min, Xiao Fei, Xi Zhi-Qin
机构信息
Department of Neurology, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong Province 252000, China.
Department of Neurology, Jinan University, 601 Huangpu Avenue West, Tianhe District, Guangzhou City. Guangdong Province 510632, China.
出版信息
Iran J Basic Med Sci. 2015 Oct;18(10):1008-13.
OBJECTIVES
EMAP-like Protein 5 (EML5) is a new echinoderm microtubule-associated protein that is expressed in the central nervous system. The aim of this study was to investigate the expression profile of EML5 in the anterior temporal neocortex of patients presenting with intractable epilepsy (IE).
MATERIALS AND METHODS
Western blot assays were performed to determine EML5 expression in 36 surgically resected anterior temporal neocortices of patients with IE and eight control tissues. Immunohistochemistry and immunofluorescence were employed to explore protein expression in IE.
RESULTS
EML5 was highly expressed in both neurons and glial cells of the anterior temporal neocortex of IE patients, whereas only low levels of EML5 were detected in control brain tissues. Western blotting showed an enhanced expression of EML5 protein in the anterior temporal neocortex of IE (optical density (OD) = 1.8030 ± 0.1335/1.1852 ± 0.2253, P<0.05) compared with normal control tissues.
CONCLUSION
The results demonstrate that highly expressed EML5 in the neurons and glial cells of the cortex of patients with epilepsy is associated with microtubular dysfunction after frequent and recurrent seizures.
目的
类EMAP蛋白5（EML5）是一种新的棘皮动物微管相关蛋白，在中枢神经系统中表达。本研究旨在调查EML5在难治性癫痫（IE）患者颞叶新皮质前部的表达情况。
材料与方法
采用蛋白质免疫印迹法检测36例IE患者手术切除的颞叶新皮质前部组织及8例对照组织中EML5的表达。采用免疫组织化学和免疫荧光法研究IE中蛋白的表达情况。
结果
EML5在IE患者颞叶新皮质前部的神经元和胶质细胞中均高表达，而在对照脑组织中仅检测到低水平的EML5。蛋白质免疫印迹法显示，与正常对照组织相比，IE患者颞叶新皮质前部组织中EML5蛋白表达增强（光密度（OD）=1.8030±0.1335/1.1852±0.2253，P<0.05）。
结论
结果表明，癫痫患者皮质神经元和胶质细胞中高表达的EML5与频繁反复发作后的微管功能障碍有关。
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