Niu Yandong, Li Xiquan, Zhang Canming, Wei Ying
a Hunan Academy of Forestry , Changsha , China.
b Hunan Dongting Lake Wetland Ecosystem Research Station , Changsha , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2017 May;28(3):399-400. doi: 10.3109/19401736.2015.1127363. Epub 2016 Jan 5.
In our research, 16 sets of primers were used to amplify contiguous, overlapping segments of the complete mitochondrial DNA (mtDNA) of Odontobutis obscura. The aim of our research was to provide some important genetic background information for conservational practice of these valuable fish species. The total length of the mitochondrial genome is 16 864 bp and deposited in the GenBank with accession numbers KT438552. The gene arrangement and transcriptional direction were similar to other bony fishes which contained 37 genes (13 protein-coding genes, 2 ribosomal RNA, and 22 transfer RNAs) and a major non-coding control region. The nucleotide skewness for the coding strands of O. obscura (GC-skew = -0.26) is biased toward G and the negative GC-skew ranges from -0.51(ND2) to -0.24(CO1). The phylogenetic analysis showed that the Gobiiformes could be divided into three groups, Eleotridae, Odontobutidae and Rhyacichthyidae. The O. Obscura and O. Potamophila are sister species and clustered together.
在我们的研究中，使用了16组引物来扩增暗纹东方鲀完整线粒体DNA（mtDNA）的连续重叠片段。我们研究的目的是为这些珍贵鱼类物种的保护实践提供一些重要的遗传背景信息。线粒体基因组全长16864bp，并已存入GenBank，登录号为KT438552。基因排列和转录方向与其他硬骨鱼类相似，包含37个基因（13个蛋白质编码基因、2个核糖体RNA和22个转移RNA）以及一个主要的非编码控制区。暗纹东方鲀编码链的核苷酸偏斜度（GC偏斜度=-0.26）偏向于G，负GC偏斜度范围从-0.51（ND2）到-0.24（CO1）。系统发育分析表明，鲈形目可分为三个类群，塘鳢科、沙塘鳢科和溪鳢科。暗纹东方鲀和河川沙塘鳢是姐妹物种，聚在一起。
