Yu Fan, Yu Juhua, Zhou Yi, Yan Jinpeng, Fang Yanhong, Wang Wenjun, Yang Zhong
a Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization , Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences , Wuxi , China.
b Department of Biotechnology and Environmental Science , Changsha University , Changsha , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4706-4707. doi: 10.3109/19401736.2015.1106511. Epub 2015 Dec 18.
In our research, 16 sets of primers were used to amplify contiguous, overlapping segments of the complete mitochondrial DNA (mtDNA) of Ameiurus melas. The total length of the mitochondrial genome is 16 512 bp and deposited in the GenBank with accession no. KT804702. The gene arrangement and transcriptional direction were similar to other bony fishes which contained 37 genes (13 protein-coding genes, 2 ribosomal RNA, and 22 transfer RNAs) and a major non-coding control region. The G contents were lowest (17.36%) and the nucleotide skewness for the coding strands of A. melas (GC-skew = -0.24) is biased toward G and the negative GC-skew ranges from -0.44 (ND6) to -0.13 (CO1). The phylogenetic studies indicate that A. melas and the Ictalurus punctatus are cluster together, they are sister group. However, the phylogenetic relationship of other Cyprininae has some differences, such as Pangasianodon gigas and Silurus asotus, which need further research.
在我们的研究中，使用了16组引物来扩增黑真鮰完整线粒体DNA（mtDNA）的连续重叠片段。线粒体基因组全长16512 bp，并以登录号KT804702存入GenBank。基因排列和转录方向与其他硬骨鱼类相似，包含37个基因（13个蛋白质编码基因、2个核糖体RNA和22个转运RNA）以及一个主要的非编码控制区。G含量最低（17.36%），黑真鮰编码链的核苷酸偏斜度（GC偏斜=-0.24）偏向G，负GC偏斜范围从-0.44（ND6）到-0.13（CO1）。系统发育研究表明，黑真鮰和斑点叉尾鮰聚在一起，它们是姐妹群。然而，其他鲤科鱼类的系统发育关系存在一些差异，如巨无齿鷶和鲶鱼，这需要进一步研究。
