Phylogenetic study of Ameiurus melas based on complete mitochondrial DNA sequence.

In our research, 16 sets of primers were used to amplify contiguous, overlapping segments of the complete mitochondrial DNA (mtDNA) of Ameiurus melas. The total length of the mitochondrial genome is 16 512 bp and deposited in the GenBank with accession no. KT804702. The gene arrangement and transcriptional direction were similar to other bony fishes which contained 37 genes (13 protein-coding genes, 2 ribosomal RNA, and 22 transfer RNAs) and a major non-coding control region. The G contents were lowest (17.36%) and the nucleotide skewness for the coding strands of A. melas (GC-skew = -0.24) is biased toward G and the negative GC-skew ranges from -0.44 (ND6) to -0.13 (CO1). The phylogenetic studies indicate that A. melas and the Ictalurus punctatus are cluster together, they are sister group. However, the phylogenetic relationship of other Cyprininae has some differences, such as Pangasianodon gigas and Silurus asotus, which need further research.