Zhang Hong, Zeng Da, Xu Youhou, Zhang Yanqiu, Fang Huaiyi, Xu Lili, Gong Bin
Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Guangxi Colleges and Universities Key Laboratory of Exploitation and Protection of Beibu Gulf Marine Biological Resources, Qinzhou University, Guangxi, China.
Mitochondrial DNA B Resour. 2016 Jul 10;1(1):475-476. doi: 10.1080/23802359.2016.1180551.
In our research, 17 sets of primers were used to amplify contiguous, overlapping segments of the complete mitochondrial DNA (mtDNA) of the in order to analyse the inheritance and evolutionary relationship of this species. The total length of the mitochondrial genome is 16,723 bp and deposited in the GenBank with accession numbers KU605633. The gene arrangement was similar to other bony fishes which contained 37 genes (13 protein-coding genes, 2 ribosomal RNA and 22 transfer RNAs) and a major non-coding control region. Most genes were encoded on the H-strand, except for the ND6 and 8 tRNA genes, encoding on the L-strand. The mtDNA may provide some important genetic background information for this valuable fish. The nucleotide skewness for the coding strands of (GC-skew = -0.21) is biased towards G and the negative GC-skew ranges from -0.53(ATP8) to -0.12(CO2) and the AT-Skew showed more positive varying from -0.17(ND3) to 0.26(ATP6). The phylogenetic analysis demonstrated that was clustered together with , but far away from which belongs to Beloniformes, Belonidae.
在我们的研究中，使用了17组引物来扩增该物种完整线粒体DNA（mtDNA）的连续重叠片段，以分析该物种的遗传和进化关系。线粒体基因组全长16723 bp，并以登录号KU605633保存在GenBank中。基因排列与其他硬骨鱼相似，包含37个基因（13个蛋白质编码基因、2个核糖体RNA和22个转运RNA）以及一个主要的非编码控制区。大多数基因在重链上编码，除了在轻链上编码的ND6和8个tRNA基因。线粒体DNA可能为这种珍贵鱼类提供一些重要的遗传背景信息。该物种编码链的核苷酸偏斜度（GC偏斜度=-0.21）偏向于G，负GC偏斜度范围从-0.53（ATP8）到-0.12（CO2），AT偏斜度显示出更多正值，范围从-0.17（ND3）到0.26（ATP6）。系统发育分析表明，该物种与[物种名称2]聚集在一起，但与属于颌针鱼目颌针鱼科的[物种名称3]相距甚远。
