Lu Caixia, Zhao Xinjie, Li Yan, Li Yanjie, Yuan Chengkun, Xu Fang, Meng Xiaoyu, Hou Lihui, Xu Guowang
Department Gynaecological and Obstetrical of First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
J Pharm Biomed Anal. 2016 Feb 20;120:127-33. doi: 10.1016/j.jpba.2015.12.020. Epub 2015 Dec 17.
Polycystic ovary syndrome (PCOS) is a most common, heterogeneous, complex endocrinopathy disease. Traditional Chinese medicine (TCM) has been used in the treatment of PCOS for many years. However, the mechanism underlying TCM remains obscure and challenging. In this study, 30 PCOS subjects were separated into normoinsulinemic group (NI=13) and hyperinsulinemic group (HI=17), and treated for three menstrual cycles with TCM Formula, Bushen Huatan Formula (BHF). A metabolomics approach based on ultra-high-performance liquid chromatography (UPLC) coupled with linear ion trap Orbi-trap mass spectrometer (LTQ Orbi-trap MS) is used to investigate serum metabolic changes of TCM intervention to PCOS. After BHF intervention for three menstrual cycles, the serum levels of glycerophosphorylethanolamine (GPEA), creatine, creatinine decreased in both NI and HI groups. Furthermore, in NI group, the main manifestation was the changes of phospholipid metabolism. While in HI group, lysine, phenol sulfate, phe-phe etc. decreased, and ornithine, proline, betaine, acetylcholine etc. increased. Combined with clinical biochemical data, BHF was proved effective to PCOS by reducing the inflammatory reaction and oxidative stress. This study also illustrates that the LC-MS based metabolomic approach is a helpful tool to evaluate curative effect and to understand the mechanisms of TCM.
多囊卵巢综合征（PCOS）是一种最常见的、异质性的、复杂的内分泌疾病。中医已用于治疗PCOS多年。然而，中医治疗的潜在机制仍不清楚且具有挑战性。在本研究中，30例PCOS受试者被分为正常胰岛素血症组（NI = 13）和高胰岛素血症组（HI = 17），并用中药方剂补肾化痰方（BHF）治疗三个月经周期。采用基于超高效液相色谱（UPLC）结合线性离子阱Orbitrap质谱仪（LTQ Orbitrap MS）的代谢组学方法，研究中医干预PCOS的血清代谢变化。BHF干预三个月经周期后，NI组和HI组血清甘油磷酸乙醇胺（GPEA）、肌酸、肌酐水平均下降。此外，在NI组，主要表现为磷脂代谢的变化。而在HI组，赖氨酸、硫酸苯酚、苯丙氨酸等减少，鸟氨酸、脯氨酸、甜菜碱、乙酰胆碱等增加。结合临床生化数据，证明BHF通过减轻炎症反应和氧化应激对PCOS有效。本研究还表明，基于LC-MS的代谢组学方法是评估疗效和理解中医作用机制的有用工具。
