Harvima I T, Karkola K, Harvima R J, Naukkarinen A, Neittaanmäki H, Horsmanheimo M, Fräki J E
Department of Dermatology, University of Kuopio, Finland.
Arch Dermatol Res. 1989;281(4):231-7. doi: 10.1007/BF00431056.
The distribution of tryptase in various human tissue high-salt extracts (skin, lung, pancreas, liver, kidney, and spleen) was studied. Tryptase activity was compared with tissue histamine concentration, chymase activity, and cathepsin D, and histamine-N-methyltransferase (HMT) activities. Tryptase activity, found biochemically in tissue extracts, was localized in tissue sections by an enzyme-histochemical method using peptide 4-methoxy-2-naphthylamide substrates and Fast Garnet GBC as the chromogen. The highest levels of tryptase activity were found in lung and skin extracts. Liver, kidney, and spleen extracts displayed only a little activity. The distribution of histamine was similar to that of tryptase, whereas distributions of cathepsin D and HMT were quite different from that of tryptase. High-salt extracts of lung contained no detectable chymase activity, but in skin extracts this activity was high. Using an enzyme-histochemical method, the tryptase activity in tissue sections seemed solely to be confined to cells, which were granular and Giemsa positive after the red azo dye had been removed with Tween 20. Skin and lung sections contained the highest number of positively stained cells. The inhibition properties of tryptase, found in both tissue extracts and sections, and the substrate profile in tissue sections were identical. Human leukocyte preparation was negative for tryptase when stained enzyme-histochemically. The present results suggest that tryptase in human tissues is found only in the mast cells. The enzyme seems to be identical in the various human tissues studied because the different high-salt extracts were immunologically cross-reactive when tested with a rabbit polyclonal antibody against skin tryptase.
研究了类胰蛋白酶在各种人体组织高盐提取物（皮肤、肺、胰腺、肝脏、肾脏和脾脏）中的分布情况。将类胰蛋白酶活性与组织组胺浓度、糜酶活性、组织蛋白酶D以及组胺 - N - 甲基转移酶（HMT）活性进行了比较。通过使用肽4 - 甲氧基 - 2 - 萘酰胺底物和固红GBC作为显色剂的酶组织化学方法，将在组织提取物中通过生化方法检测到的类胰蛋白酶活性定位到组织切片中。在肺和皮肤提取物中发现类胰蛋白酶活性水平最高。肝脏、肾脏和脾脏提取物仅显示出少量活性。组胺的分布与类胰蛋白酶相似，而组织蛋白酶D和HMT的分布与类胰蛋白酶有很大不同。肺的高盐提取物中未检测到糜酶活性，但皮肤提取物中的该活性较高。使用酶组织化学方法，组织切片中的类胰蛋白酶活性似乎仅局限于细胞，在用吐温20去除红色偶氮染料后，这些细胞呈颗粒状且吉姆萨染色阳性。皮肤和肺切片中阳性染色细胞数量最多。在组织提取物和切片中发现的类胰蛋白酶的抑制特性以及组织切片中的底物谱是相同的。当进行酶组织化学染色时，人白细胞制剂对类胰蛋白酶呈阴性。目前的结果表明，人体组织中的类胰蛋白酶仅存在于肥大细胞中。在所研究的各种人体组织中，这种酶似乎是相同的，因为当用针对皮肤类胰蛋白酶的兔多克隆抗体进行测试时，不同的高高盐提取物具有免疫交叉反应性。
