de Castilho Tiago Jacometo Coelho, Campos Antônio Carlos Ligocki, Mello Eneri Vieira de Souza Leite
Paraná Federal University of Paraná, Curitiba, PR.
Department of Morphophysiological Science, State University of Maringá, Maringá, PR, Brazil.
Arq Bras Cir Dig. 2015 Nov-Dec;28(4):258-61. doi: 10.1590/S0102-6720201500040010.
The use of long-chain polyunsaturated fatty acids has been studied in the context of healing and tissue regeneration mainly due to its anti-inflammatory, immunoregulatory and antioncogenic properties. Previous studies have demonstrated beneficial effects with the use of enteral immunonutrition containing various farmaconutrients such as L-arginine, omega-3, trace elements, but the individual action of each component in the healing of colonic anastomosis remains unclear.
To evaluate the influence of preoperative supplementation with omega-3 fatty acids on the healing of colonic anastomoses of well-nourished rats.
Forty Wistar adult male rats, weighing 234.4±22.3 g were used. The animals were divided into two groups: the control group received for seven days olive oil rich in omega-9 oil through an orogastric tube, while the study group received isocaloric and isovolumetric omega-3 emulsion at a dose of 100 mg/kg/day, also for seven days. Both groups were submitted to two colotomies followed by anastomosis, in the right and left colon, respectively. Parameters evaluated included changes in body weight, anastomotic complications and mortality, as well as maximum tensile strength by using a tensiometer and collagen densitometry at the anastomotic site.
There were no differences in body weight or mortality and morbidity between groups. The value of the maximum tensile strength of the control group was 1.9±0.3 N and the study group 1.7±0.2, p=0.357. There was, however, a larger amount of type I collagen deposition in the study group (p=0.0126). The collagen maturation índex was 1.74±0.71 in the control group and 1.67±0.5 in the study group; p=0,719).
Preoperative supplementation of omega-3 fatty acid in rats is associated with increased collagen deposition of type I fibers in colonic anastomoses on the 5th postoperative day. No differences were observed in the tensile strength or collagen maturation index.
长链多不饱和脂肪酸因其抗炎、免疫调节和抗癌特性，已在愈合和组织再生方面得到研究。先前的研究表明，使用含有各种营养药物（如L-精氨酸、ω-3、微量元素）的肠内免疫营养具有有益效果，但每种成分在结肠吻合口愈合中的单独作用仍不清楚。
评估术前补充ω-3脂肪酸对营养良好的大鼠结肠吻合口愈合的影响。
使用40只体重为234.4±22.3g的成年雄性Wistar大鼠。动物分为两组：对照组通过胃管接受富含ω-9油的橄榄油，持续7天，而研究组接受等热量、等体积的ω-3乳剂，剂量为100mg/kg/天，同样持续7天。两组分别在右结肠和左结肠进行两次结肠切开术并随后进行吻合。评估的参数包括体重变化、吻合口并发症和死亡率，以及使用张力计测量的最大拉伸强度和吻合部位的胶原蛋白密度测定。
两组之间的体重、死亡率和发病率没有差异。对照组的最大拉伸强度值为1.9±0.3N，研究组为1.7±0.2，p = 0.357。然而，研究组中I型胶原蛋白沉积量更大（p = 0.0126）。对照组的胶原蛋白成熟指数为1.74±0.71，研究组为1.67±0.5；p = 0.719）。
大鼠术前补充ω-3脂肪酸与术后第5天结肠吻合口中I型纤维胶原蛋白沉积增加有关。在拉伸强度或胶原蛋白成熟指数方面未观察到差异。
