Vercammen M, Gorus F, Foriers A, Segers O, Somers G, Van de Winkel M, Pipeleers D
Department of Metabolism and Endocrinology, Vrije Universiteit Brussel, Belgium.
Diabetologia. 1989 Aug;32(8):611-7. doi: 10.1007/BF00285336.
A standardized method has been developed for the assay of cell surface antibodies in IgM- and IgG-fractions from human serum. Suspensions of adult rat islet B cells, islet non-B cells, and anterior pituitary cells were used as antigen source and a cell sorter as analyser of the immunoglobulin binding to individual cells. Assay conditions were selected wherein no surface antibodies were detected in 33 control subjects younger than 20 years. In 30% of Type 1 (insulin-dependent) diabetic patients, surface antibodies were measured with rat anterior pituitary cells as well as with rat islet B cells. Binding to pituitary cells occurred with IgM- and IgG-fractions and correlated positively with IgG binding to islet B cells. At onset of the disease, the prevalence of IgM-rat anterior pituitary cell surface antibodies was higher than that of IgG-rat anterior pituitary cell surface antibodies. Cell surface antibodies were also detected in first-degree relatives of Type 1 diabetic patients, but corresponded primarily to IgM-rat anterior pituitary cell surface antibodies. It is concluded that the development of Type 1 diabetes in subjects younger than 20 years is associated with the generation of both IgM and IgG cell surface antibodies. The IgM surface antibodies may result from stimulated production of polyreactive natural autoantibodies and could precede the switch to the formation of monoreactive IgG autoantibodies. The assay of IgM cell surface antibodies can be useful in studies on the sequence of immune events in diabetes and other autoimmune disease.
已开发出一种标准化方法，用于检测人血清IgM和IgG组分中的细胞表面抗体。将成年大鼠胰岛B细胞、胰岛非B细胞和垂体前叶细胞悬液用作抗原来源，并使用细胞分选仪分析免疫球蛋白与单个细胞的结合情况。选择的检测条件是，在33名20岁以下的对照受试者中未检测到表面抗体。在30%的1型（胰岛素依赖型）糖尿病患者中，用大鼠垂体前叶细胞和大鼠胰岛B细胞检测到表面抗体。与垂体细胞的结合发生在IgM和IgG组分中，并且与IgG与胰岛B细胞的结合呈正相关。在疾病发作时，IgM-大鼠垂体前叶细胞表面抗体的患病率高于IgG-大鼠垂体前叶细胞表面抗体。在1型糖尿病患者的一级亲属中也检测到细胞表面抗体，但主要对应于IgM-大鼠垂体前叶细胞表面抗体。得出的结论是，20岁以下受试者中1型糖尿病的发生与IgM和IgG细胞表面抗体的产生有关。IgM表面抗体可能是由多反应性天然自身抗体的刺激产生所致，并且可能先于向单反应性IgG自身抗体形成的转换。IgM细胞表面抗体的检测在糖尿病和其他自身免疫性疾病的免疫事件序列研究中可能有用。
