Han Jianping, Pang Xiaohui, Liao Baosheng, Yao Hui, Song Jingyuan, Chen Shilin
Institute of Medicinal Plant Development, Chinese Academy of Medicinal Science &Peking Union Medicinal College, Beijing 100193, P.R. China.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, P.R. China.
Sci Rep. 2016 Jan 7;6:18723. doi: 10.1038/srep18723.
Adulterant herbal materials are a threat to consumer safety. In this study, we used DNA barcoding to investigate the proportions and varieties of adulterant species in traditional Chinese medicine (TCM) markets. We used a DNA barcode database of TCM (TCMD) that was established by our group to investigate 1436 samples representing 295 medicinal species from 7 primary TCM markets in China. The results indicate that ITS2 barcodes could be generated for most of the samples (87.7%) using a standard protocol. Of the 1260 samples, approximately 4.2% were identified as adulterants. The adulterant focused on medicinal species such as Ginseng Radix et Rhizoma (Renshen), Radix Rubi Parvifolii (Maomeigen), Dalbergiae odoriferae Lignum (Jiangxiang), Acori Tatarinowii Rhizoma (Shichangpu), Inulae Flos (Xuanfuhua), Lonicerae Japonicae Flos (Jinyinhua), Acanthopanacis Cortex (Wujiapi) and Bupleuri Radix (Chaihu). The survey revealed that adulterant species are present in the Chinese market, and these adulterants pose a risk to consumer health. Thus, regulatory measures should be adopted immediately. We suggest that a traceable platform based on DNA barcode sequences be established for TCM market supervision.
掺假中药材对消费者安全构成威胁。在本研究中，我们使用DNA条形码技术调查了中药市场中掺假物种的比例和种类。我们利用本团队建立的中药DNA条形码数据库（TCMD），对来自中国7个主要中药市场的代表295种药用物种的1436个样本进行了调查。结果表明，使用标准方案可为大多数样本（87.7%）生成ITS2条形码。在1260个样本中，约4.2%被鉴定为掺假品。掺假主要集中在人参、茅莓根、降香、石菖蒲、旋覆花、金银花、五加皮和柴胡等药用物种上。调查显示，中国市场存在掺假物种，这些掺假品对消费者健康构成风险。因此，应立即采取监管措施。我们建议建立一个基于DNA条形码序列的可追溯平台，用于中药市场监管。
