National Center for Natural Products Research and Research Institute of Pharmaceutical Sciences, Department of Pharmacognosy, School of Pharmacy, University of Mississippi, P.O. Box 1848, MS 38677, USA.
USDA-ARS-NPURU, P.O. Box 8048, University, MS 38677, USA.
Curr Opin Biotechnol. 2014 Feb;25:103-10. doi: 10.1016/j.copbio.2013.09.010. Epub 2013 Nov 16.
Because of the increasing demand for herbal remedies and for authentication of the source material, it is vital to provide a single database containing information about authentic plant materials and their potential adulterants. The database should provide DNA barcodes for data retrieval and similarity search. In order to obtain such barcodes, several molecular methods have been applied to develop markers that aid with the authentication and identification of medicinal plant materials. In this review, we discuss the genomic regions and molecular methods selected to provide barcodes, available databases and the potential future of barcoding using next generation sequencing.
由于对草药的需求不断增加，以及对原始材料来源的认证，因此提供一个包含有关正宗植物材料及其潜在掺杂物的信息的单一数据库是至关重要的。该数据库应为数据检索和相似性搜索提供 DNA 条码。为了获得这些条码，可以应用几种分子方法来开发有助于鉴定和识别药用植物材料的标记物。在这篇综述中，我们讨论了用于提供条码的基因组区域和分子方法、可用的数据库以及使用下一代测序进行条码的潜在未来。
