Wang Debing, Qin Yonghua, Han Jingluan, Zhang Ling, Xu Xin, Liu Xuequn, Wang Chuntai, Liu Xinqiong
Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.
Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities, Wuhan, Hubei, P.R. China; College of Life Science, South China Agricultural University, Guangzhou, Guangdong, P.R. China.
Biotechnol Biotechnol Equip. 2014 Nov 2;28(6):999-1007. doi: 10.1080/13102818.2014.978664. Epub 2014 Nov 18.
Rice blast resistance () genes-mediated resistance response depends on various resistance-related genes involved in incompatible interactions. In this work, the expression profiles of innate rice immunity related genes were examined in the mediated resistance response of true/field resistance genes. Three sets of rice near-isogenic lines (NILs) were used: the resistant NILs carrying true resistance genes in the genetic background of the susceptible cultivar Nipponbare (NB), NB-Pib, NB-Pizt, NB-Pik and NB-Pita2; NILs bearing field resistance genes in the susceptible cultivar Aichiasahi (AA) AA-pi21, Kahei (KHR). The marker gene of salicylic acid (SA) signalling was upregulated in all tested cultivars. And, (marker gene of jasmonic acid signalling) showed higher upregulation in the resistance lines with nucleotide-binding sites and leucine-rich repeat (NBS-LRR) genes , , , and than in NB and KHS. of abscisic acid (ABA) signalling may be involved in the interaction, including , , and . However, was shown to negatively regulate interaction. and were differentially expressed and strongly activated at a later stage by 48 h post-inoculation. Interestingly, there was evidence that and played an important role in the mediated response. It was shown that could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while and could be upregulated in all tested lines. The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.
稻瘟病抗性（）基因介导的抗性反应取决于参与不亲和互作的各种抗性相关基因。在本研究中，检测了水稻固有免疫相关基因在真实/田间抗性基因介导的抗性反应中的表达谱。使用了三组水稻近等基因系（NILs）：在感病品种日本晴（NB）的遗传背景中携带真实抗性基因的抗性NILs，即NB - Pib、NB - Pizt、NB - Pik和NB - Pita2；在感病品种爱知旭（AA）中携带田间抗性基因的NILs，即AA - pi21、珂黑（KHR）。水杨酸（SA）信号传导的标记基因在所有测试品种中均上调。并且，茉莉酸信号传导的标记基因在具有核苷酸结合位点和富含亮氨酸重复序列（NBS - LRR）基因、、、和的抗性品系中比在NB和KHS中上调程度更高。脱落酸（ABA）信号传导的可能参与了互作，包括、、和。然而，显示对互作起负调控作用。和在接种后48小时后期差异表达并强烈激活。有趣的是，有证据表明和在介导的反应中起重要作用。结果表明，在真实抗性品系NB - Pita2和田间抗性品系KHR中可上调，而和在所有测试品系中均可上调。这些基因的参与说明了真实/田间抗性基因介导的抗性反应中下游信号通路的复杂性。
