Fang Y, Zhu P, Li Q, Chen H, Li Y, Ren C, Hu Y, Tan Z, Gu J, Mao X
Department of Clinical Microbiology and Immunology of Southwest Hospital & the College of Medical Laboratory Science,Department of Microbiology and Biochemical Pharmacy of the College of Pharmacy,and the National Engineering Research Centre for Immunobiological Products,Third Military Medical University,Chongqing,People's Republic of China.
Department of Clinical Laboratory,People's Hospital of Sanya City,Hainan Province,People's Republic of China.
Epidemiol Infect. 2016 Jul;144(9):1917-23. doi: 10.1017/S0950268815003052. Epub 2016 Jan 8.
The phylogenetic and epidemiological relationships of 102 Burkholderia pseudomallei clinical isolates from different geographical and population sources in China were investigated by multilocus sequence typing (MLST). The MLST data were analysed using the e-BURST algorithm, and an unweighted pair-group method with arithmetic mean dendrogram was constructed based on the pair-wise differences in the allelic profiles of the strains. Forty-one sequence types (STs) were identified, of which eight were novel (ST1341, ST1345, ST1346, ST1347, ST1348, ST1349, ST1350, ST1351). No geographical-specific or host population-specific phylogenetic lineages were identified. ST46, ST50, ST55, ST58, ST70 and ST1095 predominated, but ~44% of isolates were assigned to 45 STs illustrating high genetic diversity in the strain collection. Additionally, the phylogenetic relationships of the dominant STs in China showed significant linkeage with B. pseudomallei isolates from Thailand. Analysis of the gmhD allele suggests high genetic variation in B. pseudomallei in China.
采用多位点序列分型（MLST）方法，对来自中国不同地理和人群来源的102株伯克霍尔德菌临床分离株的系统发育和流行病学关系进行了研究。使用e-BURST算法分析MLST数据，并基于菌株等位基因谱的成对差异构建了算术平均法的非加权成对组方法树状图。共鉴定出41种序列类型（STs），其中8种为新类型（ST1341、ST1345、ST1346、ST1347、ST1348、ST1349、ST1350、ST1351）。未发现地理特异性或宿主人群特异性的系统发育谱系。ST46、ST50、ST55、ST58、ST70和ST1095占主导地位，但约44%的分离株被归为45种STs，表明该菌株集合具有高度的遗传多样性。此外，中国优势STs的系统发育关系与泰国伯克霍尔德菌分离株显示出显著的联系。对gmhD等位基因的分析表明，中国伯克霍尔德菌存在高度的遗传变异。
