Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, PR China.
Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
Sci Total Environ. 2016 Mar 1;545-546:280-8. doi: 10.1016/j.scitotenv.2015.12.071. Epub 2015 Dec 31.
Haliotis diversicolor is a commercially important cultured shellfish. It is also an important marine model organism for environmental science. High temperature accompanied with hypoxia frequently induces diseases or even death to abalones. In present study, (1)H NMR spectroscopy together with pattern recognition methods was used to investigate the responses of muscle and gill of H. diversicolor to thermal and hypoxic stresses. It was found that obvious gender-, time- and tissue-specific metabolic responses were induced by thermal and hypoxic stresses. In combination with the changes of H. diversicolor in physiological features, the dual-modal stresses were suggested to mainly cause the disturbance in energy metabolism and osmotic balance in muscle and gill tissues with different mechanisms. Further, the corresponding correlation networks and metabolic pathways derived from the characteristic metabolites were used to assess the major metabolic functions of these characteristic metabolites. These findings shed some lights on the metabolic influences of environmental stresses on marine organisms.
杂色鲍是一种具有重要商业价值的养殖贝类。它也是环境科学中重要的海洋模式生物。高温伴缺氧常导致鲍鱼患病甚至死亡。本研究采用（1）H NMR 光谱结合模式识别方法，研究了杂色鲍肌肉和鳃对热胁迫和缺氧胁迫的响应。结果表明，热胁迫和缺氧胁迫诱导了明显的性别、时间和组织特异性代谢反应。结合杂色鲍生理特征的变化，双模态胁迫主要导致肌肉和鳃组织能量代谢和渗透平衡紊乱，其机制不同。此外，从特征代谢物中提取的相关网络和代谢途径用于评估这些特征代谢物的主要代谢功能。这些发现为研究环境胁迫对海洋生物的代谢影响提供了一些线索。
