Lu Jie, Shi Yanyan, Cai Shuhui, Feng Jianghua
Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, PR China.
Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
Fish Shellfish Immunol. 2017 Jan;60:265-274. doi: 10.1016/j.fsi.2016.11.051. Epub 2016 Nov 25.
Vibrio parahemolyticus is a devastating bacterial pathogen that often causes outbreak of vibriosis in abalone Haliotis diversicolor. Elucidation of metabolic mechanisms of abalones in responding to V. parahemolyticus infection is essential for controlling the epidemic. In this work, H NMR-based metabolomic techniques along with correlation and network analyses are used to investigate characteristic metabolites, as well as corresponding disturbed pathways in hepatopancreas and gill of H. diversicolor after V. parahemolyticus infection for 48 h. Results indicate that obvious gender- and tissue-specific metabolic responses are induced. Metabolic responses in female abalones are more clearly observed than those in males, which are primarily manifested in the accumulation of branched-chain amino acids and the depletion of organic osmolytes (homarine, betaine and taurine) in the infected gills of female abalones, as well as in the depletion of glutamate, branched-chain and aromatic amino acids in the infected hepatopancreases of female abalones. Moreover, based on major metabolic functions of the characteristic metabolites, we have found that V. parahemolyticus infection not only cause the disturbance in energy metabolism, nucleotide metabolism and osmotic balance, but also induce oxidative stress, immune stress and neurotoxic effect in different tissues with various mechanisms. Our study provides details of metabolic responses of abalones to V. parahemolyticus infection and will shed light on biochemical defence mechanisms of male and female hosts against pathogen infection.
副溶血性弧菌是一种具有破坏性的细菌病原体，常导致杂色鲍爆发弧菌病。阐明鲍对副溶血性弧菌感染的代谢机制对于控制疫情至关重要。在这项研究中，基于核磁共振氢谱的代谢组学技术以及相关性和网络分析被用于研究副溶血性弧菌感染48小时后杂色鲍肝胰腺和鳃中的特征性代谢物以及相应的受干扰途径。结果表明，诱导了明显的性别和组织特异性代谢反应。雌性鲍的代谢反应比雄性更明显，主要表现为雌性鲍受感染鳃中支链氨基酸的积累和有机渗透物（高甜菜碱、甜菜碱和牛磺酸）的消耗，以及雌性鲍受感染肝胰腺中谷氨酸、支链和芳香族氨基酸的消耗。此外，基于特征性代谢物的主要代谢功能，我们发现副溶血性弧菌感染不仅会导致能量代谢、核苷酸代谢和渗透平衡的紊乱，还会通过各种机制在不同组织中诱导氧化应激、免疫应激和神经毒性作用。我们的研究提供了鲍对副溶血性弧菌感染的代谢反应细节，并将揭示雄性和雌性宿主对抗病原体感染的生化防御机制。
