Harbom Lise J, Chronister William D, McConnell Michael J
Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia.
Neurosciences Graduate Program, University of Virginia School of Medicine, Charlottesville, Virginia.
Bioessays. 2016 Feb;38(2):157-61. doi: 10.1002/bies.201500097. Epub 2016 Jan 8.
A recent single cell mRNA sequencing study by Dueck et al. compares neuronal transcriptomes to the transcriptomes of adipocytes and cardiomyocytes. Single cell omic approaches such as those used by the authors are at the leading edge of molecular and biophysical measurement. Many groups are currently employing single cell sequencing approaches to understand cellular heterogeneity in cancer and during normal development. These single cell approaches also are beginning to address long-standing questions regarding nervous system diversity. Beyond an innate interest in cataloging cell type diversity in the brain, single cell neuronal diversity has important implications for neurotypic neural circuit function and for neurological disease. Herein, we review the authors' methods and findings, which most notably include evidence of unique expression profiles in some single neurons.
最近，杜克等人进行的一项单细胞mRNA测序研究将神经元转录组与脂肪细胞和心肌细胞的转录组进行了比较。作者所采用的诸如单细胞组学方法处于分子和生物物理测量的前沿。目前，许多研究小组正在采用单细胞测序方法来了解癌症和正常发育过程中的细胞异质性。这些单细胞方法也开始解决有关神经系统多样性的长期问题。除了对大脑中细胞类型多样性进行编目有着内在兴趣之外，单细胞神经元多样性对神经典型神经回路功能和神经疾病也具有重要意义。在此，我们回顾作者的方法和研究结果，其中最显著的包括一些单个神经元中独特表达谱的证据。
