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大肠杆菌K-12的recR基因座：分子克隆、DNA测序及基因产物鉴定。

The recR locus of Escherichia coli K-12: molecular cloning, DNA sequencing and identification of the gene product.

作者信息

Mahdi A A, Lloyd R G

机构信息

Department of Genetics, University of Nottingham, Queens Medical Centre, UK.

出版信息

Nucleic Acids Res. 1989 Sep 12;17(17):6781-94. doi: 10.1093/nar/17.17.6781.

DOI:10.1093/nar/17.17.6781
PMID:2674903
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318412/
Abstract

The recR gene of Escherichia coli, which is associated with recBC-independent mechanisms of recombination and DNA repair, has been located between dnaZX and htpG on a 6.4 kb EcoRI fragment of DNA that has been cloned and analysed in lambda and plasmid vectors. Nucleotide sequencing of this interval revealed two open reading frames that constitute an operon lying immediately downstream of dnaZX. The second of these two reading frames was identified as recR. It encodes a polypeptide with a predicted molecular weight of 21,965 Daltons that migrates on SDS gels as a 26 kDa protein. The first gene of the operon encodes a polypeptide of 12,015 daltons. Its function is not known.

摘要

大肠杆菌的recR基因与不依赖recBC的重组和DNA修复机制相关，它位于dnaZX和htpG之间一段6.4 kb的DNA EcoRI片段上，该片段已被克隆并在λ和质粒载体中进行分析。对该区间的核苷酸测序揭示了两个开放阅读框，它们构成了一个位于dnaZX下游紧邻的操纵子。这两个阅读框中的第二个被鉴定为recR。它编码一种预测分子量为21,965道尔顿的多肽，在SDS凝胶上迁移时表现为26 kDa的蛋白质。该操纵子的第一个基因编码一个12,015道尔顿的多肽。其功能尚不清楚。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdb3/318412/3a176b82c97e/nar00134-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdb3/318412/96cc8d8ed2c3/nar00134-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdb3/318412/d124de1b48be/nar00134-0028-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdb3/318412/3a176b82c97e/nar00134-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdb3/318412/96cc8d8ed2c3/nar00134-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdb3/318412/d124de1b48be/nar00134-0028-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdb3/318412/3a176b82c97e/nar00134-0029-a.jpg

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