大肠杆菌相邻的dnaZ和dnaX基因包含在一个连续的开放阅读框内。
The adjacent dnaZ and dnaX genes of Escherichia coli are contained within one continuous open reading frame.
作者信息
Flower A M, McHenry C S
出版信息
Nucleic Acids Res. 1986 Oct 24;14(20):8091-101. doi: 10.1093/nar/14.20.8091.
Abstract
The dnaZ and dnaX loci of Escherichia coli have been genetically defined as separate genes, both of which are essential for DNA replication (1). The 2.1 kb region of DNA that complements mutations in both genes has a maximum coding capacity of approximately 80,000 daltons. Two protein products are produced from this region with molecular weights of 77,000 and 52,000 (2,3). We have sequenced a 2.7 kb fragment containing the dnaZ and dnaX genes and determined that it contains only one open reading frame of sufficient length to encode either of these proteins. This open reading frame may encode a protein of 71,147 daltons or of 68,451 daltons depending on which potential translational initiation codon is utilized. There are two transcriptional promoters preceding the gene as well as a ribosome binding site preceding the two potential initiation codons. Both the promoters and ribosome binding sites are predicted to be weak, perhaps contributing to the low expression of these genes.
摘要
大肠杆菌的dnaZ和dnaX基因座已被遗传学定义为两个独立的基因，它们对DNA复制都是必不可少的（1）。能互补这两个基因中突变的2.1 kb DNA区域的最大编码能力约为80,000道尔顿。从该区域产生了两种蛋白质产物，分子量分别为77,000和52,000（2,3）。我们对一个包含dnaZ和dnaX基因的2.7 kb片段进行了测序，并确定它只包含一个足够长的开放阅读框，能够编码这两种蛋白质中的任何一种。根据使用哪个潜在的翻译起始密码子，这个开放阅读框可能编码一个71,147道尔顿或68,451道尔顿的蛋白质。该基因之前有两个转录启动子，以及两个潜在起始密码子之前的一个核糖体结合位点。预测启动子和核糖体结合位点都较弱，这可能导致了这些基因的低表达。
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