Takahagi M, Iwasaki H, Nakata A, Shinagawa H
Department of Experimental Chemotherapy, Osaka University, Japan.
J Bacteriol. 1991 Sep;173(18):5747-53. doi: 10.1128/jb.173.18.5747-5753.1991.
The Escherichia coli ruvC gene is involved in DNA repair and recombination and encodes an endonuclease that resolves Holliday structure in vitro. The 2.8-kb chromosomal DNA fragment that encompasses the ruvC gene and its flanking regions was cloned and sequenced. Four open reading frames were identified in the order orf17-orf26-ruvC-orf23 immediately upstream of the ruvAB operon, and their orientations are the same as the ruvAB operon, except for orf23. Proteins encoded by orf17, orf26, and ruvC (orf19) were identified by the maxicell method, and their sizes agreed with those predicted from the DNA sequences. Among the open reading frames in this region, only ruvC is involved in the repair of UV-damaged DNA. ruvC appeared to be regulated by at least two promoters, but, in contrast to the ruvAB operon, ruvC is not regulated by the SOS system as demonstrated by operon fusions.
大肠杆菌ruvC基因参与DNA修复和重组，编码一种在体外可解析霍利迪结构的核酸内切酶。克隆并测序了包含ruvC基因及其侧翼区域的2.8kb染色体DNA片段。在ruvAB操纵子上游紧邻处按orf17 - orf26 - ruvC - orf23的顺序鉴定出四个开放阅读框，除orf23外，它们的方向与ruvAB操纵子相同。通过大细胞法鉴定了由orf17、orf26和ruvC（orf19）编码的蛋白质，其大小与从DNA序列预测的大小一致。在该区域的开放阅读框中，只有ruvC参与紫外线损伤DNA的修复。ruvC似乎受至少两个启动子调控，但与ruvAB操纵子不同，如操纵子融合实验所示，ruvC不受SOS系统调控。
