Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China.
Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China.
Biosens Bioelectron. 2016 May 15;79:522-30. doi: 10.1016/j.bios.2015.12.096. Epub 2015 Dec 29.
We developed a strip biosensors array based on aptamer-modified gold nanoparticles as receptors and combined the protein-aptamer binding reaction with the streptavidin-biotin interaction as well as the sandwich format. We found that a series of protein receptors obtained a distinct response pattern to each target protein. Three proteins have been well distinguished with the naked eyes and a portable reader without mutual interference, accompanying with lower limit of detection and wider linear range. A complete set of four elementary logic gates (AND, OR, INH, and NAND) and eight combinative logic gates (AND-OR; AND-INH; OR-INH; INH-NAND; AND-OR-INH; AND-INH-NAND; OR-INH-NAND; AND-OR-INH-NAND) are thoroughly realized using this array, which could eventually be applicable to the keypad-lock system with enhanced complexity in the near future. Moreover, this array shows excellent linear relationships, anti-interference capability, real human serum samples applicability, long-term storage stability and reproducibility. All indicate that this design has very good prospects for development.
我们开发了一种基于适配体修饰的金纳米粒子的条带生物传感器阵列，将蛋白-适配体结合反应与链霉亲和素-生物素相互作用以及三明治结构结合在一起。我们发现，一系列蛋白受体对每种靶蛋白都有明显的反应模式。三种蛋白可以通过肉眼和便携式读取器进行区分，互不干扰，检测下限更低，线性范围更广。使用该阵列可以彻底实现一套完整的四个基本逻辑门（与、或、非和与非）和八个组合逻辑门（与或；与非；或非；非与非；与或非；与非非；或非非；与或非非），这在不久的将来有望应用于更复杂的键盘锁系统。此外，该阵列还表现出良好的线性关系、抗干扰能力、真实人体血清样本适用性、长期储存稳定性和重现性。所有这些都表明这种设计具有很好的发展前景。
