Faculty of Science, Kunming University of Science and Technology, Kunming 650500, China.
Biosensors (Basel). 2022 Jul 29;12(8):576. doi: 10.3390/bios12080576.
Molecular imprinting-aptamer techniques exhibit the advantages of molecular imprinting and aptamer technology. Hybrids of molecularly imprinted polymer-aptamer (MIP-aptamer) prepared by this technique have higher stability, binding affinity and superior selectivity than conventional molecularly imprinted polymers or aptamers. In recent years, molecular imprinting-aptamer technologies have attracted considerable interest for the selective recognition of target molecules in complex sample matrices and have been used in molecular recognition such as antibiotics, proteins, viruses and pesticides. This review introduced the development of molecular imprinting-aptamer-combining technologies and summarized the mechanism of MIP-aptamer formation. Meanwhile, we discussed the challenges in preparing MIP-aptamer. Finally, we summarized the application of MIP-aptamer to the molecular recognition in disease diagnosis, environmental analysis, food safety and other fields.
分子印迹-适体技术兼具分子印迹技术和适体技术的优点。该技术制备的分子印迹聚合物-适体（MIP-aptamer）杂合体比传统的分子印迹聚合物或适体具有更高的稳定性、结合亲和力和优越的选择性。近年来，分子印迹-适体技术因其在复杂样品基质中对目标分子的选择性识别而受到广泛关注，并已用于分子识别，如抗生素、蛋白质、病毒和农药。本文介绍了分子印迹-适体结合技术的发展，并总结了 MIP-aptamer 形成的机理。同时，我们讨论了制备 MIP-aptamer 所面临的挑战。最后，总结了 MIP-aptamer 在疾病诊断、环境分析、食品安全等领域的分子识别中的应用。
