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一种用于筛查贝类中副溶血性弧菌的简单、便携式电化学生物传感器。

A simple, portable, electrochemical biosensor to screen shellfish for Vibrio parahaemolyticus.

作者信息

Nordin Noordiana, Yusof Nor Azah, Abdullah Jaafar, Radu Son, Hushiarian Roozbeh

机构信息

Institute of Advanced Technology, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia.

Food Safety Research Centre, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.

出版信息

AMB Express. 2017 Dec;7(1):41. doi: 10.1186/s13568-017-0339-8. Epub 2017 Feb 15.

DOI:10.1186/s13568-017-0339-8
PMID:28205102
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5311015/
Abstract

An earlier electrochemical mechanism of DNA detection was adapted and specified for the detection of Vibrio parahaemolyticus in real samples. The reader, based on a screen printed carbon electrode, was modified with polylactide-stabilized gold nanoparticles and methylene blue was employed as the redox indicator. Detection was assessed using a microprocessor to measure current response under controlled potential. The fabricated sensor was able to specifically distinguish complementary, non-complementary and mismatched oligonucleotides. DNA was measured in the range of 2.0 × 10-2.0 × 10 M with a detection limit of 2.16 pM. The relative standard deviation for 6 replications of differential pulse voltammetry (DPV) measurement of 0.2 µM complementary DNA was 4.33%. Additionally, cross-reactivity studies against various other food-borne pathogens showed a reliably sensitive detection of the target pathogen. Successful identification of Vibrio parahaemolyticus (spiked and unspiked) in fresh cockles, combined with its simplicity and portability demonstrate the potential of the device as a practical screening tool.

摘要

一种早期的DNA检测电化学机制被采用并专门用于实际样品中副溶血性弧菌的检测。基于丝网印刷碳电极的阅读器用聚丙交酯稳定的金纳米颗粒进行了修饰，并使用亚甲基蓝作为氧化还原指示剂。使用微处理器在受控电位下测量电流响应来评估检测情况。所制备的传感器能够特异性地区分互补、非互补和错配的寡核苷酸。DNA的测量范围为2.0×10⁻².0×10 M，检测限为2.16 pM。对0.2 μM互补DNA进行差分脉冲伏安法（DPV）测量的6次重复实验的相对标准偏差为4.33%。此外，针对各种其他食源性病原体的交叉反应性研究表明，该方法能够可靠且灵敏地检测目标病原体。在新鲜蛤仔中成功鉴定出副溶血性弧菌（加标和未加标的），再加上其简单性和便携性，证明了该设备作为一种实用筛选工具的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/859a/5311015/0f16b64a2e30/13568_2017_339_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/859a/5311015/4209d04c36ab/13568_2017_339_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/859a/5311015/a780bbeb02e8/13568_2017_339_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/859a/5311015/5f19851ecc6d/13568_2017_339_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/859a/5311015/2cf4924672db/13568_2017_339_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/859a/5311015/6ab5cead542c/13568_2017_339_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/859a/5311015/0f16b64a2e30/13568_2017_339_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/859a/5311015/4209d04c36ab/13568_2017_339_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/859a/5311015/a780bbeb02e8/13568_2017_339_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/859a/5311015/5f19851ecc6d/13568_2017_339_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/859a/5311015/2cf4924672db/13568_2017_339_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/859a/5311015/6ab5cead542c/13568_2017_339_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/859a/5311015/0f16b64a2e30/13568_2017_339_Fig6_HTML.jpg

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