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RRS1中的亮氨酸拉链基序对于拟南芥双抗性蛋白复合物RPS4/RRS1的调控至关重要。

Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1.

作者信息

Narusaka Mari, Toyoda Kazuhiro, Shiraishi Tomonori, Iuchi Satoshi, Takano Yoshitaka, Shirasu Ken, Narusaka Yoshihiro

机构信息

Research Institute for Biological Sciences Okayama, Okayama 716-1241, Japan.

Faculty of Agriculture, Okayama University, Okayama 700-8530, Japan.

出版信息

Sci Rep. 2016 Jan 11;6:18702. doi: 10.1038/srep18702.

DOI:10.1038/srep18702
PMID:26750751
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4707544/
Abstract

Arabidopsis thaliana leucine-rich repeat-containing (NLR) proteins RPS4 and RRS1, known as dual resistance proteins, confer resistance to multiple pathogen isolates, such as the bacterial pathogens Pseudomonas syringae and Ralstonia solanacearum and the fungal pathogen Colletotrichum higginsianum. RPS4 is a typical Toll/interleukin 1 Receptor (TIR)-type NLR, whereas RRS1 is an atypical TIR-NLR that contains a leucine zipper (LZ) motif and a C-terminal WRKY domain. RPS4 and RRS1 are localised near each other in a head-to-head orientation. In this study, direct mutagenesis of the C-terminal LZ motif in RRS1 caused an autoimmune response and stunting in the mutant. Co-immunoprecipitation analysis indicated that full-length RPS4 and RRS1 are physically associated with one another. Furthermore, virus-induced gene silencing experiments showed that hypersensitive-like cell death triggered by RPS4/LZ motif-mutated RRS1 depends on EDS1. In conclusion, we suggest that the RRS1-LZ motif is crucial for the regulation of the RPS4/RRS1 complex.

摘要

拟南芥富含亮氨酸重复序列（NLR）的蛋白RPS4和RRS1，被称为双重抗性蛋白，可对多种病原体分离株产生抗性，如细菌病原体丁香假单胞菌和青枯雷尔氏菌以及真菌病原体菜豆炭疽菌。RPS4是典型的Toll/白细胞介素1受体（TIR）型NLR，而RRS1是一种非典型的TIR-NLR，包含一个亮氨酸拉链（LZ）基序和一个C端WRKY结构域。RPS4和RRS1以头对头的方向彼此相邻定位。在本研究中，RRS1中C端LZ基序的直接诱变导致突变体出现自身免疫反应和发育迟缓。免疫共沉淀分析表明全长RPS4和RRS1在物理上相互关联。此外，病毒诱导的基因沉默实验表明，由RPS4/LZ基序突变的RRS1触发的类过敏细胞死亡依赖于EDS1。总之，我们认为RRS1-LZ基序对于RPS4/RRS1复合物的调控至关重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34ae/4707544/8fd006a9ef7b/srep18702-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34ae/4707544/fd6421931c69/srep18702-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34ae/4707544/a423a21d1427/srep18702-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34ae/4707544/63468488b441/srep18702-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34ae/4707544/a14ed811111f/srep18702-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34ae/4707544/6bdf9e7bfada/srep18702-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34ae/4707544/7ac0d4d95068/srep18702-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34ae/4707544/8fd006a9ef7b/srep18702-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34ae/4707544/fd6421931c69/srep18702-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34ae/4707544/a423a21d1427/srep18702-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34ae/4707544/63468488b441/srep18702-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34ae/4707544/a14ed811111f/srep18702-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34ae/4707544/6bdf9e7bfada/srep18702-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34ae/4707544/7ac0d4d95068/srep18702-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34ae/4707544/8fd006a9ef7b/srep18702-f7.jpg

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2
A receptor pair with an integrated decoy converts pathogen disabling of transcription factors to immunity.一对具有整合诱饵的受体将病原体使转录因子失活转化为免疫。
Cell. 2015 May 21;161(5):1074-1088. doi: 10.1016/j.cell.2015.04.025.
3
Arabidopsis dual resistance proteins, both RPS4 and RRS1, are required for resistance to bacterial wilt in transgenic Brassica crops.
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PLoS Genet. 2017 Oct 26;13(10):e1007037. doi: 10.1371/journal.pgen.1007037. eCollection 2017 Oct.
4
DES-TOMATO: A Knowledge Exploration System Focused On Tomato Species.DES-TOMATO：一个专注于番茄属物种的知识探索系统。
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5
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