Goyal Neeru, Aliev Fazil, Latendresse Shawn J, Kertes Darlene A, Bolland John M, Byck Gayle R, Mustanski Brian, Salvatore Jessica E, Dick Danielle M
a Department of Human and Molecular Genetics , School of Medicine, Virginia Commonwealth University , Richmond , Virginia , USA.
b Virginia Institute for Psychiatric and Behavioral Genetics, Department of Psychiatry, Virginia Commonwealth University , Richmond , Virginia , USA.
Subst Abus. 2016 Jul-Sep;37(3):450-458. doi: 10.1080/08897077.2015.1134756.
Genetic and environmental factors influence substance use behaviors in youth. One of the known environmental risk factors is exposure to life stressors. The aim of this project is to study the interaction between NR3C1 and CRHBP, genes thought to be involved in stress pathways, exposure to stressful life events, and adolescent alcohol use/misuse.
The sample included 541 African American individuals (ages 13-18) from the Genes, Environment, and Neighborhood Initiative, a subset of the Mobile Youth Survey sample from whom DNA and more extensive phenotypic data were collected. Participants were selected from high-poverty neighborhoods in Mobile, Alabama, with potential exposure to a variety of extreme life stressors.
A measure of stressful life events was significantly predictive of alcohol use/misuse. In addition, this association was significantly dependent upon the number of putative risk variants at rs1715749, a single-nucleotide polymorphism (SNP) in CRHBP (P ≤ .006). There was no significant interaction between NR3C1 and stressful life events with respect to alcohol use/misuse, after taking into account multiple testing.
These findings suggest that CRHBP variants are potentially relevant for adolescent alcohol use/misuse among African American youth populations being reared within the context of stressful life events and warrant replication.
遗传和环境因素影响青少年的物质使用行为。已知的环境风险因素之一是暴露于生活应激源。本项目的目的是研究NR3C1和CRHBP之间的相互作用，这两个基因被认为参与应激途径、暴露于应激性生活事件以及青少年酒精使用/滥用。
样本包括来自基因、环境和社区倡议的541名非裔美国个体（年龄在13 - 18岁），这是移动青年调查样本的一个子集，从中收集了DNA和更广泛的表型数据。参与者从阿拉巴马州莫比尔的高贫困社区中选取，可能暴露于各种极端生活应激源。
应激性生活事件的一种测量方法显著预测了酒精使用/滥用。此外，这种关联显著依赖于CRHBP中一个单核苷酸多态性（SNP）rs1715749处推定风险变体的数量（P≤0.006）。在考虑多重检验后，NR3C1和应激性生活事件在酒精使用/滥用方面没有显著的相互作用。
这些发现表明CRHBP变体可能与生活在应激性生活事件背景下的非裔美国青年人群中的青少年酒精使用/滥用相关，值得重复研究。
