具有增强的环境光稳定性的3-三氟甲基-3-芳基二氮杂环丙烷光标记物。
3-Trifluoromethyl-3-aryldiazirine photolabels with enhanced ambient light stability.
作者信息
Kumar Arun Babu, Tipton Jeremiah D, Manetsch Roman
机构信息
Department of Chemistry, University of South Florida, Tampa, Florida 33620, USA.
Proteomics and Mass Spectrometry Core Facility, University of South Florida, Tampa, Florida 33620, USA.
出版信息
Chem Commun (Camb). 2016 Feb 14;52(13):2729-32. doi: 10.1039/c5cc09518b.
Abstract
Ambient light stable 3-trifluoromethyl-3-aryldiazirine photolabels are developed via stabilization of the strained three membered diazirine ring by replacing the phenyl ring with electron withdrawing heterocyclic rings. Photolabeling studies reveal that these ambient light stable photolabels are equally efficient in photolabeling target proteins as the traditional 3-trifluoromethyl-3-phenyldiazirine and found to significantly increase the aqueous solubility of the photoaffinity labels.
摘要
通过用吸电子杂环取代苯环来稳定张力三元二氮杂环丙烷环，从而开发出环境光稳定的3-三氟甲基-3-芳基二氮杂环丙烷光标记物。光标记研究表明，这些环境光稳定的光标记物在光标记靶蛋白方面与传统的3-三氟甲基-3-苯基二氮杂环丙烷同样有效，并且发现它们能显著提高光亲和标记物的水溶性。
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