Rothko K, Kickler T S, Clay M E, Johnson R J, Stroncek D F
Department of Pathology and Laboratory Medicine, Johns Hopkins University School of Medicine, Baltimore.
Blood. 1989 Oct;74(5):1698-703.
We characterized neutrophil autoantigens using an immunoblotting technique with antibodies obtained from patients with autoimmune neutropenia. These results were correlated with serologic characterization of the antibodies, using indirect immunofluorescence and leukoagglutination. Of the 17 sera immunoblotted, 16 showed discrete bands in the molecular weight range of 30 to 112. Three patients with Felty's syndrome reacted with an antigenic target of 80 to 84 Kd molecular mass, a finding not seen in any of the other patients studied. By serologic testing, none of the autoimmune sera showed serologic specificity for any known neutrophil-specific alloantigen. Using an anti-NA-1 serum, we identified antigenic targets at 40, 50, and 101 Kd in both NA-1-positive and NA-1-negative neutrophils. Ten of 17 autoimmune sera showed reactivity in this corresponding range. These studies demonstrate that immunoblotting may be used to identify antigenic targets in autoimmune neutropenia and may suggest a specificity of these antibodies not definable by serologic techniques. Correlation of immunoblot reactivity with disease states associated with immune neutropenia may be useful in the study of the pathogenesis of the different forms of autoimmune neutropenia.
我们使用免疫印迹技术，利用从自身免疫性中性粒细胞减少症患者获得的抗体，对中性粒细胞自身抗原进行了表征。这些结果与抗体的血清学表征相关，采用间接免疫荧光和白细胞凝集法。在17份进行免疫印迹的血清中，16份在分子量范围为30至112处显示出离散条带。三名费尔蒂综合征患者与分子量为80至84 Kd的抗原靶点发生反应，这一发现在所研究的其他患者中均未出现。通过血清学检测，没有一份自身免疫血清对任何已知的中性粒细胞特异性同种抗原显示出血清学特异性。使用抗NA-1血清，我们在NA-1阳性和NA-1阴性中性粒细胞中均鉴定出了40、50和101 Kd的抗原靶点。17份自身免疫血清中有10份在该相应范围内显示出反应性。这些研究表明，免疫印迹可用于识别自身免疫性中性粒细胞减少症中的抗原靶点，并可能提示这些抗体具有血清学技术无法定义的特异性。免疫印迹反应性与与免疫性中性粒细胞减少症相关的疾病状态的相关性，可能有助于研究不同形式的自身免疫性中性粒细胞减少症的发病机制。
