Kim Dong-Hwi, Chang Yoon Hyuk, Kwak Hae-Soo
Department of Food Science and Technology, Sejong University, Seoul, 143-747, Korea.
Department of Food and Nutrition, Kyung Hee University, Seoul 130-701, Korea.
Korean J Food Sci Anim Resour. 2014;34(1):49-56. doi: 10.5851/kosfa.2014.34.1.49. Epub 2014 Feb 28.
The present study was carried out to investigate the effects of nanopowdered peanut sprout-added Caciocavallo cheese (NPCC) on the prevention and treatment of rheumatoid arthritis in DBA/IJ mice immunized with type II collagen. After the induction of arthritis, the mice were being divided into five groups: (1) normal, no immunization; (2) CIA, collagen-induced arthritis; (3) MTX, collagen-induced arthritis treated with methotrexate (0.3 mg/kg body weight); (4) CC, collagen-induced arthritis treated with Caciocavallo cheese (0.6 g/d); (5) NPCC, collagen-induced arthritis treated with nanopowdered peanut sprout-added Caciocavallo cheese (0.6 g/d). Nanopowdered peanut sprout was ranged from 300 to 350 nm, while regular powdered peanut sprouts were ranged from 50 to 150 μm. The NPCC group had considerable reductions of clinical scores and paw thicknesses at the end of experiment as compared to the CIA group. In the serum analysis, the TNF-α, IL-1β, IL- 6 and IgG1 levels in the NPCC group have decreased by 69.4, 75.9, 66.6, and 61.9%, respectively, when compared to the CIA group. The histological score and spleen index of the NPCC group were significantly lower than the CIA group. In conclusion, the feeding NPCC method could delay and/or prevent the rheumatoid arthritis in the collagen-induced arthritis mouse model. Based on this study, nanopowdered peanut sprouts could be applied to various functional cheeses.
本研究旨在探讨添加纳米花生芽的卡乔卡瓦洛奶酪（NPCC）对用II型胶原蛋白免疫的DBA/IJ小鼠类风湿性关节炎的预防和治疗效果。诱导关节炎后，将小鼠分为五组：（1）正常组，未免疫；（2）CIA组，胶原诱导的关节炎组；（3）MTX组，用甲氨蝶呤（0.3mg/kg体重）治疗的胶原诱导的关节炎组；（4）CC组，用卡乔卡瓦洛奶酪（0.6g/d）治疗的胶原诱导的关节炎组；（5）NPCC组，用添加纳米花生芽的卡乔卡瓦洛奶酪（0.6g/d）治疗的胶原诱导的关节炎组。纳米花生芽的粒径范围为300至350nm，而普通花生芽粉的粒径范围为50至150μm。与CIA组相比，NPCC组在实验结束时临床评分和爪厚度显著降低。血清分析中，与CIA组相比，NPCC组的TNF-α、IL-1β、IL-6和IgG1水平分别下降了69.4%、75.9%、66.6%和61.9%。NPCC组的组织学评分和脾脏指数显著低于CIA组。综上所述，喂食NPCC的方法可以延缓和/或预防胶原诱导的关节炎小鼠模型中的类风湿性关节炎。基于本研究，纳米花生芽可应用于各种功能性奶酪。
