Mohs F E
University of Wisconsin School of Medicine, Madison.
Dermatol Clin. 1989 Oct;7(4):609-11.
Excising cancer in successive layers and examining the entire underside of each layer in the microscope by the systematic use of frozen sections ensure eradication of the neoplasm, including its "silent" outgrowths, and this is accomplished with the maximal sparing of normal tissue. Fresh-tissue micrographic surgery is used for most cancers, but the fixed-tissue technique is safer for melanomas because all incisions are made through fixed (killed) tissues, avoiding the danger of disseminating highly transplantable melanoma cells from transecting silent outgrowths or clinically invisible satellites. Although the essential concept was very simple, more than a half century of continuing development was required to bring the method to its present status; further progress is an exciting prospect.
通过系统使用冰冻切片，逐层切除癌症组织并在显微镜下检查每一层的整个底面，可确保根除肿瘤，包括其“隐匿性”生长部分，且能最大程度地保留正常组织。新鲜组织显微手术适用于大多数癌症，但对于黑色素瘤，固定组织技术更安全，因为所有切口均通过固定（已杀死）的组织进行，避免了因横切隐匿性生长部分或临床不可见的卫星灶而播散高转移性黑色素瘤细胞的风险。尽管其基本概念非常简单，但经过半个多世纪的不断发展，该方法才达到目前的水平；进一步的进展前景令人兴奋。
