Huang Guo-min, Tian Xiao-feng, Fang Xue-dong, Ji Fu-jian
Department of General Surgery, the China-Japan Union Hospital of Jilin University, China.
Department of General Surgery, the China-Japan Union Hospital of Jilin University, China.
Chemosphere. 2016 Mar;147:188-94. doi: 10.1016/j.chemosphere.2015.12.080. Epub 2016 Jan 7.
While bisphenol F (BPF) has been frequently detected in various environmental compartments, limited information is available on its effect on thyroid endocrine system. In the present study, zebrafish (Danio rerio) embryos were exposed to 0.2, 2, 20, and 200 μg/L of BPF from 2 h post-fertilization (hpf) to 144 hpf. The whole-body content of thyroid hormones, thyroid-stimulating hormone (TSH), and transcription of genes belonging to the hypothalamic-pituitary-thyroid (HPT) axis were investigated. BPF exposure resulted in alterations of both T3 and T4 contents, increased the ratios of T3/T4, demonstrating thyroid endocrine disruption. Moreover, TSH content was significantly induced in a concentration-dependent manner after exposure to BPF. The increased gene transcription of dio2 might assist to degrade increased T3 contents. Treatment with BPF also significantly increased transcription of genes involved in thyroid hormone regulation (crh) and synthesis (nis and tg) as a compensatory mechanism for the decrease of T4 contents. However, the gene encoding protein involved in TH transport (ttr) was transcriptionally significantly down-regulated after exposure to BPF. Taken together, these results suggest that BPF alters the transcription of genes involved in the HPT axis as well as changes whole-body contents of thyroid hormones and TSH in zebrafish embryos/larvae, thus causing an endocrine disruption of the thyroid system.
虽然双酚F（BPF）已在各种环境介质中频繁检测到，但其对甲状腺内分泌系统影响的信息有限。在本研究中，斑马鱼（Danio rerio）胚胎从受精后2小时（hpf）至144 hpf暴露于0.2、2、20和200μg/L的BPF中。研究了甲状腺激素、促甲状腺激素（TSH）的全身含量以及下丘脑-垂体-甲状腺（HPT）轴相关基因的转录情况。BPF暴露导致T3和T4含量均发生改变，T3/T4比值升高，表明甲状腺内分泌受到干扰。此外，暴露于BPF后，TSH含量以浓度依赖的方式显著升高。dio2基因转录增加可能有助于降解升高的T3含量。BPF处理还显著增加了参与甲状腺激素调节（crh）和合成（nis和tg）的基因转录，作为T4含量降低的一种补偿机制。然而，暴露于BPF后，参与甲状腺激素转运的蛋白质编码基因（ttr）的转录显著下调。综上所述，这些结果表明BPF改变了斑马鱼胚胎/幼体中HPT轴相关基因的转录，以及甲状腺激素和TSH的全身含量，从而导致甲状腺系统的内分泌干扰。
