Luzón Mónica, Domínguez-González Julia, Soto-Carrión Ana María, Alunda José María, Fuente Concepción de la
Department of Animal Health, Veterinary Faculty, Complutense University of Madrid, Avenida de Puerta de Hierro s/n, 28040 Madrid, Spain.
Int J Parasitol Parasites Wildl. 2015 Nov 11;4(3):396-400. doi: 10.1016/j.ijppaw.2015.11.001. eCollection 2015 Dec.
Red deer (Cervus elaphus) from a National Wildlife Reserve near Toledo in central Spain were surveyed for Sarcocystis infection. A total of 61 deer were examined. Tissue compression and histology were used to examine samples from diaphragm and heart from each animal included in the study, and results from the two techniques and the two tissues were compared to determine the tissue and technique that provide the most accurate measure of prevalence and intensity. Prevalence and intensity were then compared between calves, yearlings and adults. Sarcocystis was detected in 59 (97%) of the 61 deer. Comparison between tissues showed that (a) prevalence based on histology was similar for heart and diaphragm, (b) prevalence based on compression was significantly higher for heart than for diaphragm and (c) intensity was significantly higher for heart than for diaphragm, regardless of the technique used. Comparison between techniques showed that (a) both techniques rendered similar prevalences and intensities of Sarcocystis infection with heart samples and (b) both techniques were not comparable with diaphragm samples (compression rendered lower prevalence but higher intensity than histology). Together these data suggest that heart is the preferable tissue for estimating prevalence and intensity, regardless of the technique used. A preliminary species identification of isolated cysts from three animals showed two morph types, corresponding to Sarcocystis cervicanis (syn. S. cf. grueneri; S. wapiti) in the heart and diaphragm of three animals and S. hjorti, only in the diaphragm of two animals. Given the different location of those morph types, both heart and diaphragm should be sampled and preferably assessed using histology to most reliably detect infection. Based on histology of heart, prevalence and intensity of Sarcocystis were significantly lower in calves than in yearlings or adults.
对来自西班牙中部托莱多附近一个国家野生动物保护区的马鹿（Cervus elaphus）进行了肉孢子虫感染调查。共检查了61只鹿。采用组织压片法和组织学方法对研究中每只动物的膈肌和心脏样本进行检查，并比较两种技术和两种组织的结果，以确定能提供最准确患病率和感染强度测量值的组织和技术。然后比较犊鹿、一岁龄鹿和成年鹿之间的患病率和感染强度。在61只鹿中有59只（97%）检测到肉孢子虫。组织间比较显示：（a）基于组织学的心脏和膈肌患病率相似；（b）基于压片法的心脏患病率显著高于膈肌；（c）无论使用何种技术，心脏的感染强度均显著高于膈肌。技术间比较显示：（a）两种技术对心脏样本得出的肉孢子虫感染患病率和感染强度相似；（b）两种技术对膈肌样本无可比性（压片法得出的患病率较低但感染强度高于组织学方法）。这些数据共同表明，无论使用何种技术，心脏都是估计患病率和感染强度的首选组织。对三只动物分离囊肿的初步种属鉴定显示有两种形态类型，分别对应三只动物心脏和膈肌中的鹿肉孢子虫（同物异名：S. cf. grueneri；S. wapiti）以及仅在两只动物膈肌中的霍氏肉孢子虫。鉴于这些形态类型的位置不同，应同时采集心脏和膈肌样本，最好采用组织学方法进行评估，以最可靠地检测感染情况。基于心脏组织学检查，犊鹿的肉孢子虫患病率和感染强度显著低于一岁龄鹿或成年鹿。
