[Dynamic Changes of Nucleosides and Nucleobases in Different Harvest Periods of Polygoni Multiflori Radix by UPLC-QTRAP-MS/MS].

OBJECTIVE

To analyze the dynamic changes of nucleosides and nucleobases in Polygoni Multiflori Radix harvested in different periods.

METHODS

UPLC-QTRAP-MS/MS method was applied for the analysis of nine kinds of nucleosides and nucleobases in Polygoni Multiflori Radix.

RESULTS

The content of uridine, adenine, guanosine and cytidine was higher in Polygoni Multiflori Radix harvested in different periods and assumed some difference. The trends of nucleosides and nucleobases from Polygoni Multiflori Radix according to the peak valley shape changed. The highest contents of them were in December.

CONCLUSION

The accumulation of nucleosides and nucleobases in Polygoni Multiflori Radix is closely related to its growth cycle. It is found to be basically the same as that obtained when the herb is collected during the conventional collecting time.