干燥性角结膜炎诱导小鼠模型中不同浓度人羊水的效果

Outcomes of different concentrations of human amniotic fluid in a keratoconjunctivitis sicca-induced mouse model.

作者信息

Quinto Guilherme G, Castro-Combs Juan, Li Li, Gupta Navin, Campos Mauro, Behrens Ashley

机构信息

Department of Ophthalmology, The Wilmer Ophthalmological Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Department of Ophthalmology, Vision Institute, Federal University of São Paulo, São Paulo, Brazil.

出版信息

Int Ophthalmol. 2016 Oct;36(5):643-50. doi: 10.1007/s10792-016-0180-0. Epub 2016 Jan 14.

DOI:10.1007/s10792-016-0180-0

PMID:26767655

Abstract

To compare the effects of different concentrations of topical human amniotic fluid (HAF) in a mouse model of dry eye, forty C57BL/6 mice were divided into 4 treatment groups: 20 % HAF, 50 % HAF, 100 % HAF, and isotonic salt solution (control). Dry eye was induced by an injection of botulinum toxin B into the lacrimal gland. Tear production, ocular surface fluorescein staining, and blink rate were evaluated in each mouse at 5 time points during a 4-week period. Goblet cell density was assessed in stained histological sections. Regarding tear production, 20, 50, and 100 % HAF groups were all different from the control group (P < 0.001) at week 1. However, there were no statistically significant differences between the 20, 50, and 100 % HAF groups. At week 2, 20, 50, and 100 % HAF groups had significant improvement in staining score and were significantly different from the control group (P = 0.047, P = 0.005, and P = 0.001, respectively). No difference in spontaneous blink rate was observed between groups, at any time point. Goblet cell density was significantly decreased in the control group compared to the HAF treatment groups. All tested concentrations of topical HAF were effective and superior than the control in this keratoconjunctivitis sicca-induced mouse model. Further studies are needed to evaluate the effects of HAF on the human ocular surface.

摘要

为了比较不同浓度的局部应用人羊水（HAF）在干眼小鼠模型中的效果，将40只C57BL/6小鼠分为4个治疗组：20% HAF组、50% HAF组、100% HAF组和等渗盐溶液组（对照组）。通过向泪腺注射肉毒杆菌毒素B诱导干眼。在4周期间的5个时间点对每只小鼠的泪液分泌、眼表荧光素染色和眨眼频率进行评估。在染色的组织学切片中评估杯状细胞密度。关于泪液分泌，在第1周时，20%、50%和100% HAF组均与对照组不同（P < 0.001）。然而，20%、50%和100% HAF组之间无统计学显著差异。在第2周时，20%、50%和100% HAF组的染色评分有显著改善，且与对照组有显著差异（分别为P = 0.047、P = 0.005和P = 0.001）。在任何时间点，各组之间均未观察到自发眨眼频率的差异。与HAF治疗组相比，对照组的杯状细胞密度显著降低。在这个干燥性角结膜炎诱导的小鼠模型中，所有测试浓度的局部应用HAF均有效且优于对照组。需要进一步研究来评估HAF对人眼表的影响。