Zeng Ni, Tong Bei, Zhang Xinyu, Dou Yannong, Wu Xin, Xia Yufeng, Dai Yue, Wei Zhifeng
Department of Pharmacology of Chinese Materia Medica, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing, 210009, China.
Drug Dev Res. 2015 Dec;76(8):463-73. doi: 10.1002/ddr.21282. Epub 2015 Oct 7.
Morin, a flavonoid isolated from Morus alba L. (Moraceae), possesses anti-inflammatory, antiangiogenic among other biological activities. This study investigated its effect on type II collagen-induced arthritis (CIA) in rats and explored the underlying mechanisms in view of synovial angiogenesis. Morin administered po attenuated arthritic progression as indicated by reduction of arthritis scores and paw swelling. It also markedly reduced serum levels of the proinflammatory cytokines, tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6), but increased the level of anti-inflammatory cytokine interleukin-10, and ameliorated histopathological changes of joints. Morin markedly inhibited expression of CD31, vascular endothelial growth factor (VEGF), and basic fibroblast growth factor in synovial membrane tissues, and decreased serum levels of VEGF in CIA rats. In vitro, morin markedly inhibited VEGF-induced migration and tube formation in human umbilical vein endothelial cells. These results indicate that morin had antirheumatoid potential, and its mechanism might be associated with inhibition of synovial angiogenesis.
桑色素是从桑科植物桑（Morus alba L.）中分离出的一种黄酮类化合物，具有抗炎、抗血管生成等多种生物学活性。本研究调查了其对大鼠Ⅱ型胶原诱导性关节炎（CIA）的影响，并从滑膜血管生成的角度探讨了潜在机制。口服给予桑色素可减轻关节炎进展，表现为关节炎评分降低和 paw 肿胀减轻。它还显著降低了促炎细胞因子肿瘤坏死因子α（TNF-α）和白细胞介素-6（IL-6）的血清水平，但提高了抗炎细胞因子白细胞介素-10的水平，并改善了关节的组织病理学变化。桑色素显著抑制滑膜组织中CD31、血管内皮生长因子（VEGF）和成纤维细胞生长因子的表达，并降低CIA大鼠血清中VEGF的水平。在体外，桑色素显著抑制VEGF诱导的人脐静脉内皮细胞迁移和管腔形成。这些结果表明桑色素具有抗类风湿潜力，其机制可能与抑制滑膜血管生成有关。
