Chart H, Scotland S M, Smith H R, Rowe B
Division of Enteric Pathogens, Central Public Health Laboratory, Colindale, London.
J Clin Pathol. 1989 Sep;42(9):973-6. doi: 10.1136/jcp.42.9.973.
Twenty four sera from patients with haemolytic uraemic syndrome or haemorrhagic colitis and healthy controls were examined for antibodies to the lipopolysaccharide (LPS) of Escherichia coli O157. Faecal specimens from these patients were also examined for Vero cytotoxin producing E coli (VTEC) by DNA probes, and for faecal Vero cytotoxin. Eight patients with faecal E coli O157:H7 gave a strong antibody response to O157 LPS, shown by immunoblotting and an enzyme linked immunosorbent assay. Six symptomatic patients without evidence of faecal VTEC also gave a strong antibody response to O157 LPS. Sera from the remaining five patients and five healthy controls did not contain antibodies to E coli O157. The results suggest that the testing of sera from patients with haemorrhagic colitis or haemolytic uraemic syndrome by ELISA or immunoblot would prove valuable in addition to the established procedures for detecting VTEC, using DNA probes and testing for faecal Vero cytotoxin.
检测了24份溶血性尿毒综合征或出血性结肠炎患者以及健康对照者的血清，以检测其针对大肠杆菌O157脂多糖（LPS）的抗体。还通过DNA探针检测了这些患者的粪便标本中产生志贺毒素的大肠杆菌（VTEC），并检测了粪便中的志贺毒素。8例粪便中含有大肠杆菌O157:H7的患者通过免疫印迹和酶联免疫吸附测定显示出对O157 LPS的强烈抗体反应。6例有症状但无粪便VTEC证据的患者也对O157 LPS产生了强烈抗体反应。其余5例患者和5例健康对照者的血清中未含有针对大肠杆菌O157的抗体。结果表明，除了使用DNA探针检测VTEC和检测粪便志贺毒素的既定程序外，通过ELISA或免疫印迹检测出血性结肠炎或溶血性尿毒综合征患者的血清将被证明是有价值的。
