Boqué C, Pujol-Moix N, Linde M A, Murcia C, Guanyabens C, Soler J
Servei d'Hematologia, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
J Clin Pathol. 1989 Sep;42(9):982-4. doi: 10.1136/jcp.42.9.982.
Monoclonal anti-actin was used as a marker of megakaryocytes in Zenker's fixed, paraffin wax embedded bone marrow tissue, using an immunoperoxidase staining method. Twenty bone marrow samples were studied, including controls, and different myeloproliferative and myelodysplastic syndromes. The results were compared with those obtained using factor VIII related antigen (F VIII RAg) immunolabelling. Anti-actin is as good a marker for megakaryocytes as anti-FVIIIRAg and is potentially clinically useful when morphological identification is difficult, when bone marrow aspiration is unsuccessful, or when quantitative evaluation of tissue sections is required.
采用免疫过氧化物酶染色法，将单克隆抗肌动蛋白用作在岑克尔氏固定、石蜡包埋的骨髓组织中巨核细胞的标志物。研究了20份骨髓样本，包括对照样本以及不同的骨髓增殖性和骨髓发育异常综合征样本。将结果与使用因子VIII相关抗原（F VIII RAg）免疫标记所获得的结果进行比较。抗肌动蛋白与抗FVIIIRAg一样，都是巨核细胞的良好标志物，在形态学识别困难、骨髓穿刺不成功或需要对组织切片进行定量评估时，可能具有临床应用价值。
