Wang Hao, Li Li, Zhang Yin, Pan Shu-Chao, Chen An-Qiang, Qian Wei-Dong
Department of Neurology, The First Affiliated Hospital of Bengbu Medical College Bengbu 233004, China.
Int J Clin Exp Med. 2015 Oct 15;8(10):19126-31. eCollection 2015.
This study aims to investigate the changes of immune status and significance in patients with Guillain-Barré syndrome (GBS).
The proportion of CD4(+)CD25(+)CD127(-) regulatory T cells in peripheral blood before immunotherapy for 41 patients with GBS (including 29 classic type and 12 variant type) and 42 normal control patients (healthy volunteers) were evaluated by flow cytometry. And molybdenum three phenol red method was used to detect cerebrospinal fluid protein content of 28 patients with GBS (including 19 with classic type and 9 with variant type).
Compared with healthy control group, the CD4(+)CD25(+)CD127(-) of GBS group had obvious difference (P<0.05). Of which, the CD4(+)CD25(+)CD127(-) regulatory T cells of the classic GBS group had no significant changes compared with the variant group (P>0.05), as well as the cerebrospinal fluid protein content between classic and variant GBS groups. The decrease of the proportion of CD4(+)CD25(+)CD127(-) regulatory T cells suggested abnormal expression of immune function in GBS patients.
The decrease of GBS regulatory T cell number or function indicated that the immune regulatory T cells mediated imbalance of immune regulation involved in the pathogenesis of GBS.
本研究旨在探讨吉兰 - 巴雷综合征（GBS）患者免疫状态的变化及其意义。
采用流式细胞术评估41例GBS患者（包括29例经典型和12例变异型）免疫治疗前外周血中CD4(+)CD25(+)CD127(-)调节性T细胞的比例，以及42例正常对照患者（健康志愿者）。采用钼酸三酚红法检测28例GBS患者（包括19例经典型和9例变异型）的脑脊液蛋白含量。
与健康对照组相比，GBS组CD4(+)CD25(+)CD127(-)有明显差异（P<0.05）。其中，经典GBS组与变异组的CD4(+)CD25(+)CD127(-)调节性T细胞无显著变化（P>0.05），经典型和变异型GBS组之间的脑脊液蛋白含量也无显著变化。CD4(+)CD25(+)CD127(-)调节性T细胞比例的降低提示GBS患者免疫功能表达异常。
GBS调节性T细胞数量或功能的降低表明免疫调节性T细胞介导的免疫调节失衡参与了GBS的发病机制。
