Kim Hyemin, Bae Seyeon, Kwon Kil-Young, Hwang Young-Il, Kang Jae Seung, Lee Wang Jae
Department of Anatomy, Seoul National University College of Medicine, Seoul, Korea.; Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul, Korea.
Department of Anatomy, Seoul National University College of Medicine, Seoul, Korea.
Anat Cell Biol. 2015 Dec;48(4):244-50. doi: 10.5115/acb.2015.48.4.244. Epub 2015 Dec 21.
The mechanism of Western medicine that is commonly used for pain relief is well-known. However, very little is known for oriental herbs, and even less is known for mixture of the two. We investigated the combinational effect of 3 kinds of oriental herbs, usually used for the control of headache, and acetaminophen to relieve headache in microglia cell line, BV2. Lipopolysaccharide (LPS) stimulation induced to produce nitrite and increased the expression of inflammation-related factors like inducible nitric oxide synthase and cyclooxygenase-2 (COX-2) in murine microglia cell line, BV2. Oriental herbs such as Angelica tenuissima, Angelica dahurica, and Scutellaria baicalensis reduced the production of nitric oxide and the expression of COX-2. Moreover, a treatment of acetaminophen combined with oriental herbs was more decreased the COX-2 expression, and its product, prostaglandin E2 production in BV2 cells. Therefore, a combined treatment of oriental herbs such as A. tenuissima, A. dahurica, and S. baicalensis and Western medicine like acetaminophen has a synergistic effect on the decrease of LPS-induced inflammation in microglia.
常用于缓解疼痛的西药机制广为人知。然而，对于东方草药的了解却非常少，对于二者混合物的了解更是少之又少。我们研究了3种常用于控制头痛的东方草药与对乙酰氨基酚联合使用对小胶质细胞系BV2缓解头痛的作用。脂多糖（LPS）刺激可诱导小鼠小胶质细胞系BV2产生亚硝酸盐，并增加炎症相关因子如诱导型一氧化氮合酶和环氧化酶-2（COX-2）的表达。当归、白芷和黄芩等东方草药可减少一氧化氮的产生和COX-2的表达。此外，对乙酰氨基酚与东方草药联合处理可进一步降低BV2细胞中COX-2的表达及其产物前列腺素E2的产生。因此，当归、白芷和黄芩等东方草药与对乙酰氨基酚等西药联合治疗对减轻LPS诱导的小胶质细胞炎症具有协同作用。
