Department of Biophysics, Institute of Physics, Maria Curie-Sklodowska University, Lublin, Poland; Chair of Soil Science, Environmental Chemistry and Hydrology, Faculty of Biology and Agriculture, University of Rzeszow, Rzeszow, Poland.
Department of Biophysics, Institute of Physics, Maria Curie-Sklodowska University, Lublin, Poland.
Nanomedicine. 2016 May;12(4):1095-1103. doi: 10.1016/j.nano.2015.12.378. Epub 2016 Jan 7.
High antifungal activity is reported, in comparison with commercially available products, of a novel hybrid system based on silver nanoparticles synthesized using a popular antifungal macrocyclic polyene amphotericin B (AmB) acting both as a reducing and stabilizing/capping agent. The synthesis reaction proceeds in an alkaline environment which prevents aggregation of AmB itself and promotes nanoparticle formation. The innovative approach produces monodisperse (PDI=0.05), AmB-coated silver nanoparticles (AmB-AgNPs) with the diameter ~7nm. The products were characterized using imaging (electron microscopy) and spectroscopic (UV-vis and infrared absorption, dynamic light scattering and Raman scattering) methods. The nanoparticles were tested against Candida albicans, Aspergillus niger and Fusarium culmorum species. For cytotoxicity studies CCD-841CoTr and THP-1 cell lines were used. Particularly high antifungal activity of AmB-AgNPs is interpreted as the result of synergy between the antifungal activity of amphotericin B and silver antimicrobial properties (Ag(+) ions release).
Amphotericin B (AmB) is a common agent used for the treatment against severe fungal infections. In this article, the authors described a new approach in using a combination of AmB and silver nanoparticles, in which the silver nanoparticles were synthesized and stabilized by AmB. Experimental data confirmed synergistic antifungal effects between amphotericin B and silver. This novel synthesis process could potentially be important in future drug development and fabrication.
与市售产品相比，一种新型的基于银纳米粒子的混合系统具有很高的抗真菌活性，该系统使用一种流行的抗真菌大环多烯两性霉素 B（AmB）作为还原剂和稳定/封端剂。合成反应在碱性环境中进行，这可以防止 AmB 本身的聚集，并促进纳米粒子的形成。这种创新的方法产生了单分散（PDI=0.05）的、被 AmB 包裹的银纳米粒子（AmB-AgNPs），其直径约为 7nm。使用成像（电子显微镜）和光谱（UV-vis 和红外吸收、动态光散射和拉曼散射）方法对产物进行了表征。这些纳米粒子被测试了对白色念珠菌、黑曲霉和尖孢镰刀菌的抗真菌活性。为了进行细胞毒性研究，使用了 CCD-841CoTr 和 THP-1 细胞系。AmB-AgNPs 的高抗真菌活性被解释为两性霉素 B 的抗真菌活性和银的抗菌特性（Ag+离子释放）之间协同作用的结果。
两性霉素 B（AmB）是一种常用于治疗严重真菌感染的常用药物。在这篇文章中，作者描述了一种使用两性霉素 B 和银纳米粒子结合的新方法，其中银纳米粒子是由两性霉素 B 合成和稳定的。实验数据证实了两性霉素 B 和银之间的协同抗真菌作用。这种新的合成工艺在未来的药物开发和制造中可能具有重要意义。
